Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,850,891	G→A	Q68* (CAA→TAA)	ilvN ←	acetohydroxy acid synthase I subunit IlvN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,850,891	0	G	A	100.0%	89.0 / NA	23	Q68* (CAA→TAA)	ilvN	acetohydroxy acid synthase I subunit IlvN
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/12);  total (11/12)

ACTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTGGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGCCA  >  NC_000913/3850846‑3850936                                              |                                              aCTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCt                                            >  1:1885514/1‑49 (MQ=255)  cTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCtt                                           <  1:1777487/49‑1 (MQ=255)      ttACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCTTatca                                       <  1:1459082/49‑1 (MQ=255)      ttACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCTTatca                                       <  1:2031672/49‑1 (MQ=255)          gCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCTTATCATCTg                                   >  1:620226/1‑49 (MQ=255)              cACTTTCACGACATCTTCCAGCTTATCGATTTAGCTTATCATCTGCTcc                               <  1:294019/49‑1 (MQ=255)                    cACGACATCTTCCAGCTTATCGATTTAGCTTATCATCTGCTCCAGACg                          <  1:1500563/48‑1 (MQ=255)                     aCGACATCTTCCAGCTTATCGATTTAGCTTATCATCTGCTCCAGACGCt                        <  1:190804/49‑1 (MQ=255)                      cGACATCTTCCAGCTTATCGATTTAGCTTATCATCTGCTCCAGACGCTg                       <  1:1372307/49‑1 (MQ=255)                      cGACATCTTCCAGCTTATCGATTTAGCTTATCATCTGCTCCAGACGCTg                       >  1:1118678/1‑49 (MQ=255)                        aCATCTTCCAGCTTATCGATTTAGCTTATCATCTGCTCCAGACGCTGgt                     >  1:94393/1‑49 (MQ=255)                           tCTTCCAGCTTATCGATTTAGCTTATCATCTGCTCCAGACGCTGgtcgt                  >  1:593506/1‑49 (MQ=255)                               ccAGCTTATCGATTTAGCTTATCATCTGCTCCAGACGCTGgtcgtc                 <  1:545526/46‑1 (MQ=255)                                cAGCTTATCGATTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCAtt              >  1:38284/1‑48 (MQ=255)                                cAGCTTATCGATTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCATTg             <  1:280626/49‑1 (MQ=255)                                 aGCTTATCGATTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGa            <  1:1980743/49‑1 (MQ=255)                                  gCTTATCGATTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGAc           >  1:2047599/1‑49 (MQ=255)                                   cTTATCGATTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGAc           >  1:602874/1‑48 (MQ=255)                                      aTCGATTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAg        >  1:1471601/1‑48 (MQ=255)                                       tCGATTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAg        >  1:1144963/1‑47 (MQ=255)                                         gATTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGc    <  1:1249430/49‑1 (MQ=255)                                          aTTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGc    >  1:63328/1‑48 (MQ=255)                                           tttAGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGCCa  <  1:1585059/49‑1 (MQ=255)                                              |                                              ACTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTGGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGCCA  >  NC_000913/3850846‑3850936

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑