Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,871,692	C→T	R13R (CGG→CGA)	dgoT ←	putative D‑galactonate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,871,692	0	C	T	100.0%	103.6 / NA	26	R13R (CGG→CGA)	dgoT	putative D‑galactonate transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/9);  total (17/9)

AAATGACTACCGTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAG  >  NC_000913/3871649‑3871736                                            |                                             aaaTGACTACCGTAATAAAGATCATCACCAGCGTCAGATAACGTCGACg                                         <  1:1040647/49‑1 (MQ=255)    tGACTACCGTAATAAAGATCATCACCAGCGTCAGATAACGTCGACGccc                                      >  1:2010391/1‑49 (MQ=255)     gACTACCGTAATAAAGATCATCACCAGCGTCAGATAACGTCGACGcccc                                     >  1:927071/1‑49 (MQ=255)       cTACCGTAATAAAGATCATCACCAGCGTCAGATAACGTCGACGCCCCgg                                   >  1:135552/1‑49 (MQ=255)          ccGTAATAAAGATCATCACCAGCGTCAGATAACGTCGACGCCCCGGCtt                                >  1:1168675/1‑49 (MQ=255)            gTAATAAAGATCATCACCAGCGTCAGATAACGTCGACGCCCCGGCTTtg                              >  1:1788052/1‑49 (MQ=255)               ataaAGATCATCACCAGCGTCAGATAACGTCGACGCCCCGGCTTtgctg                           >  1:1295665/1‑49 (MQ=255)               ataaAGATCATCACCAGCGTCAGATAACGTCGACGCCCCGGCTTtgct                            <  1:1452933/48‑1 (MQ=255)               ataaAGATCATCACCAGCGTCAGATAACGTCGACGCCCCGGCTTtgct                            <  1:1767434/48‑1 (MQ=255)                taaAGATCATCACCAGCGTCAGATAACGTCGACGCCCCGGCTTtgctgc                          <  1:1101131/49‑1 (MQ=255)                taaAGATCATCACCAGCGTCAGATAACGTCGACGCCCCGGCTTtgctg                           >  1:1065755/1‑48 (MQ=255)                 aaaGATCATCACCAGCGTCAGATAACGTCGACGCCCCGGCTTTGCTGCa                         >  1:544209/1‑49 (MQ=255)                    gATCATCACCAGCGTCAGATAACGTCGACGCCCCGGCTTTGCTGCAtt                       >  1:244306/1‑48 (MQ=255)                    gATCATCACCAGCGTCAGATAACGTCGACGCCCCGGCTTTGCTGCAtt                       >  1:573945/1‑48 (MQ=255)                     atcatcACCAGCGTCAGATAACGTCGACGCCCCGGCTTTGCTGCATTaa                     >  1:876239/1‑49 (MQ=255)                     atcatcACCAGCGTCAGATAACGTCGACGCCCCGGCTTTGCTGCATTaa                     >  1:38801/1‑49 (MQ=255)                       catcaCCAGCGTCAGATAACGTCGACGCCCCGGCTTTGCTGCATTAACg                   >  1:1963721/1‑49 (MQ=255)                        atcaCCAGCGTCAGATAACGTCGACGCCCCGGCTTTGCTGCATTAACgg                  <  1:1102468/49‑1 (MQ=255)                         tcaCCAGCGTCAGATAACGTCGACGCCCCGGCTTTGCTGCATTAACggg                 <  1:1508864/49‑1 (MQ=255)                           aCCAGCGTCAGATAACGTCGACGCCCCGGCTTTGCTGCATTAACGGGaa               >  1:595252/1‑49 (MQ=255)                              aGCGTCAGATAACGTCGACGCCCCGGCTTTGCTGCATTAACGGGAatat            >  1:522253/1‑49 (MQ=255)                               gCGTCAGATAACGTCGACGCCCCGGCTTTGCTGCATTAACGGGAATATc           <  1:953848/49‑1 (MQ=255)                                    aGATAACGTCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATa       <  1:1674235/48‑1 (MQ=255)                                      aTAACGTCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGc     >  1:212621/1‑48 (MQ=255)                                      aTAACGTCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGc     >  1:177734/1‑48 (MQ=255)                                         aCGTCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAg  <  1:689562/48‑1 (MQ=255)                                            |                                             AAATGACTACCGTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAG  >  NC_000913/3871649‑3871736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑