Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	380,022	(G)10→9	intergenic (‑141/+47)	frmR ← / ← yaiO	DNA‑binding transcriptional repressor FrmR/outer membrane protein YaiO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	380,013	0	G	.	100.0%	86.9 / NA	19	intergenic (‑132/+56)	frmR/yaiO	DNA‑binding transcriptional repressor FrmR/outer membrane protein YaiO
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (14/5);  total (14/5)

GAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGG  >  NC_000913/379966‑380055                                                |                                           gagaATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgg                                           <  1:1886085/49‑1 (MQ=255) gagaATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgg                                           <  1:145590/49‑1 (MQ=255)  agaATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAggg                                          >  1:62266/1‑49 (MQ=255)  agaATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAggg                                          >  1:1485034/1‑49 (MQ=255)     aTAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgggggg                                       >  1:1201299/1‑49 (MQ=255)      tAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAggggggg                                      >  1:2047391/1‑49 (MQ=255)      tAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAggggggg                                      >  1:352045/1‑49 (MQ=255)       aaaaTTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgggggggg                                     >  1:875419/1‑49 (MQ=255)       aaaaTTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgggggggg                                     >  1:1282650/1‑49 (MQ=255)        aaaTTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgggggggg                                     <  1:248612/48‑1 (MQ=255)         aaTTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgggggggggc                                   >  1:466198/1‑48 (MQ=255)               tCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATc                             <  1:724064/48‑1 (MQ=255)               tCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCg                            <  1:1617332/49‑1 (MQ=255)                 cGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCgg                           <  1:614362/48‑1 (MQ=255)                      aaTGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAggg                      >  1:1084724/1‑47 (MQ=255)                       aTGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAggg                      >  1:1082006/1‑47 (MQ=255)                           ggTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGgcc                >  1:565529/1‑49 (MQ=255)                           ggTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGgcc                >  1:851782/1‑49 (MQ=255)                            gTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGgccg               <  1:54091/49‑1 (MQ=255)                              cGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGgccgcc             >  1:1022141/1‑49 (MQ=255)                               ggAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGccc            >  1:1938575/1‑49 (MQ=255)                               ggAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGccc            >  1:1155530/1‑49 (MQ=255)                                gAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGcccc           >  1:886594/1‑49 (MQ=255)                                gAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGcccc           >  1:1772250/1‑49 (MQ=255)                                 aaaaCAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCg          >  1:107129/1‑49 (MQ=255)                                   aaCAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGa        >  1:1787387/1‑49 (MQ=255)                                   aaCAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGa        >  1:82513/1‑49 (MQ=255)                                    aCAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGAt       <  1:1883738/49‑1 (MQ=255)                                      aaaGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAg     >  1:1158913/1‑49 (MQ=255)                                         gagGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcgg  >  1:64229/1‑49 (MQ=255)                                                |                                           GAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGG  >  NC_000913/379966‑380055

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑