Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	765,191	Δ1 bp	coding (685/723 nt)	mngR ←	DNA‑binding transcriptional repressor MngR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	765,189	0	T	.	100.0%	127.4 / NA	28	coding (687/723 nt)	mngR	DNA‑binding transcriptional repressor MngR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (18/10);  total (18/10)

TTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACAC  >  NC_000913/765143‑765233                                               |                                             ttttttGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAAtta                                            >  1:934358/1‑48 (MQ=255)      tgtgCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGt                                       >  1:1130112/1‑48 (MQ=38)      tgtgCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGt                                       <  1:1464867/48‑1 (MQ=38)      tgtgCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGt                                       >  1:240498/1‑48 (MQ=38)      tgtgCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGt                                       >  1:1725505/1‑48 (MQ=38)        tgCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCAt                                    >  1:1321449/1‑49 (MQ=38)        tgCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCAt                                    >  1:736338/1‑49 (MQ=38)          cTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATc                                   <  1:138734/48‑1 (MQ=38)              tCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTAt                              <  1:766080/49‑1 (MQ=38)               cgcgATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTAt                              >  1:1458904/1‑48 (MQ=38)                gcgATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTAtt                             <  1:293319/48‑1 (MQ=38)                gCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTA                            >  1:1775306‑M1/1‑49 (MQ=255)                 cGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTA                            >  1:32475‑M1/1‑48 (MQ=255)                   atgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtag                         >  1:1800851‑M1/1‑46 (MQ=255)                     gatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagc                        >  1:994699‑M1/1‑44 (MQ=255)                     gatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagc                        >  1:267895‑M1/1‑44 (MQ=255)                      atTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaa                      <  1:1637100‑M1/49‑7 (MQ=255)                      atTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagca                       <  1:1383574‑M1/48‑6 (MQ=255)                         ttCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatc                    >  1:783188‑M1/1‑40 (MQ=255)                         ttCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatc                    >  1:1213362‑M1/1‑40 (MQ=255)                          tCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatca                   >  1:1134296‑M1/1‑39 (MQ=255)                           cGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaa                 >  1:158448‑M1/1‑38 (MQ=255)                           cGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaa                  <  1:651624‑M1/48‑11 (MQ=255)                                  cTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagc          >  1:1693633‑M1/1‑31 (MQ=255)                                  cTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagc          >  1:830272‑M1/1‑31 (MQ=255)                                  cTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagc          >  1:1572439‑M1/1‑31 (MQ=255)                                     tCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagcca        <  1:864530‑M1/48‑21 (MQ=255)                                        gggTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccacata    <  1:282756‑M1/49‑25 (MQ=255)                                          gTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccacatatg  <  1:1788695‑M1/49‑27 (MQ=255)                                               |                                             TTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACAC  >  NC_000913/765143‑765233

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑