Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	972,866	Δ1 bp	coding (536/780 nt)	elyC ←	envelope biogenesis factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	972,863	0	G	.	100.0%	64.1 / NA	16	coding (539/780 nt)	elyC	envelope biogenesis factor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (9/7);  total (9/7)

AGATCATCGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTC  >  NC_000913/972816‑972906                                                |                                            aGATCATCGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAgg                                            >  1:884182/1‑49 (MQ=255)   atcatcGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAgggc                                          >  1:576940/1‑48 (MQ=255)   atcatcGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAgggc                                          >  1:1965710/1‑48 (MQ=255)   atcatcGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAgggc                                          <  1:1655157/49‑2 (MQ=255)    tcatcGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAgggcg                                         >  1:974197/1‑47 (MQ=255)    tcatcGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAgggcg                                         >  1:1778354/1‑47 (MQ=255)         gCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAA‑GGGCGTCAc                                    >  1:1589383/1‑48 (MQ=39)             gcgGCAGATGTGATGCGGAGGTCACCAGCAGGAAA‑GGGCGTCACCAATc                               >  1:1233260/1‑49 (MQ=39)             gcgGCAGATGTGATGCGGAGGTCACCAGCAGGAAA‑GGGCGTCACCAATc                               <  1:1224147/49‑1 (MQ=39)               ggCAGATGTGATGCGGAGGTCACCAGCAGGAAA‑GGGCGTCACCAATCGc                             >  1:1380856/1‑49 (MQ=39)               ggCAGATGTGATGCGGAGGTCACCAGCAGGAAA‑GGGCGTCACCAATCGc                             >  1:1373910/1‑49 (MQ=39)                gCAGATGTGATGCGGAGGTCACCAGCAGGAAA‑GGGCGTCACCAATCGcc                            <  1:1510361/49‑1 (MQ=39)                 cAGATGTGATGCGGAGGTCACCAGCAGGAAA‑GGGCGTCACCAATCGCCt                           <  1:2085546/49‑1 (MQ=39)                 cAGATGTGATGCGGAGGTCACCAGCAGGAAA‑GGGCGTCACCAATCGCCt                           >  1:1217239/1‑49 (MQ=39)                      gtgATGCGGAGGTCACCAGCAGGAAA‑GGGCGTCACCAATCGCCTGttt                       >  1:293711/1‑48 (MQ=39)                            cGGAGGTCACCAGCAGGAAA‑GGGCGTCACCAATCGCCTGTTTCActgct                >  1:1313213/1‑49 (MQ=39)                               aGGTCACCAGCAGGAAA‑GGGCGTCACCAATCGCCTGTTTCActgctg               >  1:1335161/1‑47 (MQ=39)                                  tCACCAGCAGGAAA‑GGGCGTCACCAATCGCCTGTTTCACTGCTGCAGct          <  1:480458/49‑1 (MQ=39)                                   cACCAGCAGGAAA‑GGGCGTCACCAATCGCCTGTTTCACTGCTGCAGctt         >  1:729100/1‑49 (MQ=39)                                    aCCAGCAGGAAA‑GGGCGTCACCAATCGCCTGTTTCACTGCTGCAGctt         <  1:590845/48‑1 (MQ=39)                                     ccAGCAGGAAA‑GGGCGTCACCAATCGCCTGTTTCACTGCTGCAGcttct       <  1:617227/49‑1 (MQ=39)                                          agGAAA‑GGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCttcttcttc  <  1:66607/49‑1 (MQ=39)                                                |                                            AGATCATCGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTC  >  NC_000913/972816‑972906

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑