Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,948,805	(G)6→7	coding (1165/1521 nt)	yifB ←	putative magnesium chelatase YifB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,948,799	1	.	G	100.0%	73.9 / NA	20	G391R (GGC→CGC)	yifB	putative magnesium chelatase YifB
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (7/13);  total (7/13)

AACGGTGGCGCTGCTTTCTCCCGGCACTACCGTTTTACTCAAAATGCC‑GGGGGGTGGTAATGGGATCTCCAGTGAGAGATCGAAGCGGTC  >  NC_000913/3948752‑3948841                                                 |                                           aaCGGTGGCGCTGCTTTCTCCCGGCACTACCGTTTTACTCAAAATGCC‑g                                           >  1:1929344/1‑49 (MQ=255)  aCGGTGGCGCTGCTTTCTCCCGGCACTACCGTTTTACTCAAAATGCC‑gg                                          <  1:1952215/49‑1 (MQ=255)    ggTGGCGCTGCTTTCTCCCGGCACTACCGTTTTACTCAAAATGCC‑gggg                                        <  1:1620808/49‑1 (MQ=255)    ggTGGCGCTGCTTTCTCCCGGCACTACCGTTTTACTCAAAATGCC‑gggg                                        <  1:780879/49‑1 (MQ=255)                     gcGGCACTACCGTTTTACTCAAAATGCCGGGGGGGTGGTAATGGGAtc                         <  1:1120605/47‑1 (MQ=255)                     ccGGCACTACCGTTTTACTCAAAATGCCGGGGGGGTGGTAATGGGa                           <  1:1602262/46‑1 (MQ=255)                     ccGGCACTACCGTTTTACTCAAAATGCCGGGGGGGTGGTAATGGGa                           <  1:288984/46‑1 (MQ=255)                     ccGGCACTACCGTTTTACTCAAAATGCCGGGGGGGTGGTAATGGGAtct                        <  1:484128/49‑1 (MQ=255)                     ccGGCACTACCGTTTTACTCAAAATGCCGGGGGGGTGGTAATGGGAt                          <  1:383124/47‑1 (MQ=255)                     ccGGCACTACCGTTTTACTCAAAATGCCGGGGGGGTGGTAATGGGAt                          <  1:297162/47‑1 (MQ=255)                       ggCACTACCGTTTTACTCAAAATGCCGGGGGGGTGGTAATGGGAtctc                       >  1:803321/1‑48 (MQ=255)                        gCACTACCGTTTTACTCAAAATGCCGGGGGGGTGGTAATGGGAtctc                       >  1:2018805/1‑47 (MQ=255)                             aCCGTTTTACTCAAAATGCCGGGGGGGTGGTAATGGGATCTCCAGt                   >  1:1666061/1‑46 (MQ=255)                              ccGTTTTACTCAAAATGCCGGGGGGGTGGTAATGGGATCTCCAGTgaga               <  1:669201/49‑1 (MQ=255)                              ccGTTTTACTCAAAATGCCGGGGGGGTGGTAATGGGATCTCCAGTga                 <  1:241100/47‑1 (MQ=255)                                 ttttACTCAAAATGCCGGGGGGGTGGTAATGGGATCTCCAGTga                 >  1:242027/1‑44 (MQ=255)                                  tttACTCAAAATGCCGGGGGGGTGGTAATGGGATCTCCAGTga                 >  1:13106/1‑43 (MQ=255)                                     aCTCAAAATGCCGGGGGGGTGGTAATGGGATCTCCAGTGAGAGATCGa         <  1:1547668/48‑1 (MQ=255)                                       tCAAAATGCCGGGGGGGTGGTAATGGGATCTCCAGTGAGAGATCGAAGc      >  1:1787577/1‑49 (MQ=255)                                        cAAAATGCCGGGGGGGTGGTAATGGGATCTCCAGTGAGAGATCGAAGCg     >  1:827587/1‑49 (MQ=255)                                         aaaaTGCCGGGGGGGTGGTAATGGGATCTCCAGTGAGAGATCGAAGc      <  1:1536687/47‑1 (MQ=255)                                         aaaaTGCCGGGGGGGTGGTAATGGGATCTCCAGTGAGAGATCGAAGCgg    <  1:2050993/49‑1 (MQ=255)                                         aaaaTGCCGGGGGGGTGGTAATGGGATCTCCAGTGAGAGATCGAAGCg     <  1:1648012/48‑1 (MQ=255)                                           aaTGCCGGGGGGGTGGTAATGGGATCTCCAGTGAGAGATCGAAGCGGTc  <  1:761576/49‑1 (MQ=255)                                                 |                                           AACGGTGGCGCTGCTTTCTCCCGGCACTACCGTTTTACTCAAAATGCC‑GGGGGGTGGTAATGGGATCTCCAGTGAGAGATCGAAGCGGTC  >  NC_000913/3948752‑3948841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑