Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	12,086	C→T	intergenic (‑300/‑77)	yaaI ← / → dnaK	DUF2541 domain‑containing protein YaaI/chaperone protein DnaK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	12,086	0	C	T	100.0%	149.7 / NA	39	intergenic (‑300/‑77)	yaaI/dnaK	DUF2541 domain‑containing protein YaaI/chaperone protein DnaK
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/25);  total (14/25)

GTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACCACA  >  NC_000913/12042‑12131                                             |                                              gtagtCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGTAGtt                                           <  1:817114/49‑1 (MQ=255)    gtCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGTAGTTGaa                                        <  1:939039/49‑1 (MQ=255)    gtCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGTAGTTGaa                                        <  1:301801/49‑1 (MQ=255)    gtCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGTAGTTGa                                         <  1:1644006/48‑1 (MQ=255)       aaCCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGTAGTTGAAAc                                      <  1:1322005/48‑1 (MQ=255)       aaCCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGTAGTTGAAAc                                      <  1:998855/48‑1 (MQ=255)       aaCCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGTAGTTGAAAc                                      <  1:801877/48‑1 (MQ=255)          cGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGa                                  <  1:1310809/49‑1 (MQ=255)          cGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGa                                  <  1:2038521/49‑1 (MQ=255)           gCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGAc                                 >  1:652224/1‑49 (MQ=255)           gCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGAc                                 <  1:723597/49‑1 (MQ=255)            cAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGACg                                <  1:639079/49‑1 (MQ=255)             agtgagtgAGTCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGACGt                               >  1:19084/1‑49 (MQ=255)              gtgagtgaGTCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGACGtt                              <  1:590491/49‑1 (MQ=255)               tgagtgagTCTGCAAAAAAATGAGATTGGGTAGTTGAAACCAGACGttt                             <  1:831068/49‑1 (MQ=255)                 agtgagtCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGACGTTTc                            <  1:1956332/48‑1 (MQ=255)                 agtgagtCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGACGTTTc                            <  1:275856/48‑1 (MQ=255)                 agtgagtCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGACGTTTc                            <  1:482396/48‑1 (MQ=255)                 agtgagtCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGACGTTTCg                           >  1:1844315/1‑49 (MQ=255)                   tgagtCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGACGTTTCGcc                         <  1:2046049/49‑1 (MQ=255)                   tgagtCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGACGTTTCGcc                         >  1:137722/1‑49 (MQ=255)                     agtCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGACGTTTCGcccc                       >  1:181615/1‑49 (MQ=255)                      gtCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGACGTTTCGCCCCt                      <  1:598755/49‑1 (MQ=255)                      gtCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGACGTTTCGCCCCt                      <  1:1686590/49‑1 (MQ=255)                       tCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGACGTTTCGCCCCTa                     >  1:1068638/1‑49 (MQ=255)                        cTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGACGTTTCGCCCCTAt                    >  1:1160110/1‑49 (MQ=255)                           cAAAAAAATGAAATTGGGTAGTTGAAACCAGACGTTTCGCCCCTAt                    <  1:284670/46‑1 (MQ=255)                           cAAAAAAATGAAATTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTa                  >  1:95250/1‑48 (MQ=255)                            aaaaaaaTGAAATTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTACa                <  1:650339/49‑1 (MQ=255)                              aaaaaTGAAATTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTACAGa              <  1:534416/49‑1 (MQ=255)                                aaaTGAAATTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTACAGACt            >  1:663146/1‑49 (MQ=255)                                aaaTGAAATTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTACAGACt            >  1:728132/1‑49 (MQ=255)                                     aaaTTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTcaca        <  1:1351502/48‑1 (MQ=255)                                     aaaTTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACaa       <  1:539651/49‑1 (MQ=255)                                     aaaTTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACaa       >  1:865884/1‑49 (MQ=255)                                       aTTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAAcc     <  1:321635/49‑1 (MQ=255)                                         tGGGTAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACca    >  1:371795/1‑48 (MQ=255)                                          gggTAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACcaca  >  1:328436/1‑49 (MQ=255)                                          gggTAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACcaca  >  1:1723550/1‑49 (MQ=255)                                             |                                              GTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACCACA  >  NC_000913/12042‑12131

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑