Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,926,696	C→T	G103D (GGC→GAC)	asnC ←	DNA‑binding transcriptional dual regulator AsnC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,926,696	0	C	T	100.0%	82.7 / NA	21	G103D (GGC→GAC)	asnC	DNA‑binding transcriptional dual regulator AsnC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/8);  total (13/8)

GATCGAACGGCACATCACTTTTATAAAGATGCTGTAGTGGCCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCT  >  NC_000913/3926656‑3926741                                         |                                              gatcgaACGGCACATCACTTTTATAAAGATGCTGTAGTGGTCGGTtgt                                        <  1:1863489/48‑1 (MQ=255)    cgaACGGCACATCACTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTaa                                    >  1:355365/1‑49 (MQ=255)     gaACGGCACATCACTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTaa                                    >  1:1041080/1‑48 (MQ=255)     gaACGGCACATCACTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTaa                                    >  1:1846028/1‑48 (MQ=255)      aaCGGCACATCACTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTAATa                                  <  1:1653556/49‑1 (MQ=255)        cGGCACATCACTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTAATAgg                                >  1:2032230/1‑49 (MQ=255)          gCACATCACTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCt                              <  1:974542/49‑1 (MQ=255)            acaTCACTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCtt                             >  1:205750/1‑48 (MQ=255)            acaTCACTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCtt                             >  1:670033/1‑48 (MQ=255)            acaTCACTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTc                            <  1:1946668/49‑1 (MQ=255)             caTCACTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTCa                           <  1:1326467/49‑1 (MQ=255)                 aCTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGa                       >  1:2051890/1‑49 (MQ=255)                    tttATAAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACtt                    >  1:1799574/1‑49 (MQ=255)                     ttATAAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACt                     <  1:367526/47‑1 (MQ=255)                       ataAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACTtcat                 >  1:1952877/1‑49 (MQ=255)                        taAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACTtcat                 >  1:141061/1‑48 (MQ=255)                          aaGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACTtcatc                >  1:1000809/1‑47 (MQ=255)                             aTGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGc           <  1:1720474/49‑1 (MQ=255)                                cTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCttt        >  1:1162735/1‑49 (MQ=255)                                cTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCtt         <  1:813272/48‑1 (MQ=255)                                      tGGTCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCt  >  1:932380/1‑49 (MQ=255)                                         |                                              GATCGAACGGCACATCACTTTTATAAAGATGCTGTAGTGGCCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCT  >  NC_000913/3926656‑3926741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑