Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,979,769	C→T	A390V (GCG→GTG)	wzyE →	enterobacterial common antigen polymerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,979,769	0	C	T	100.0%	119.1 / NA	31	A390V (GCG→GTG)	wzyE	enterobacterial common antigen polymerase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/14);  total (17/14)

CCTAATCGCTATAAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGCGATCTTCAATATGATCGTGCTGGCGCGTGAAGGGCTGGATTCGTT  >  NC_000913/3979723‑3979814                                               |                                              ccTAATCGCTATAAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGTGa                                             >  1:1906155/1‑49 (MQ=255)  cTAATCGCTATAAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGTGAt                                            >  1:1430869/1‑49 (MQ=255)     aTCGCTATAAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGTGATc                                           <  1:1174401/47‑1 (MQ=255)      tCGCTATAAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGTGATCtt                                         >  1:709840/1‑48 (MQ=255)      tCGCTATAAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGTGATCTTc                                        <  1:1095495/49‑1 (MQ=255)        gCTATAAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGTGATCTTCaa                                      >  1:1175808/1‑49 (MQ=255)         cTATAAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGTGATCTTCAat                                     <  1:1813693/49‑1 (MQ=255)          tataAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGTGATCTTCAata                                    >  1:1939833/1‑49 (MQ=255)            taAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGTGATCTTCAATATg                                  <  1:998680/49‑1 (MQ=255)            taAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGTGATCTTCAATATg                                  <  1:824324/49‑1 (MQ=255)              aaGCTGCGATATTGCACAGTTTCTGCTTTGGGGTGATCTTCAATATGAt                                <  1:889063/49‑1 (MQ=255)              aaGCTGCGATATTGCACAGTTTCTGCTTTGGGGTGATCTTCAATATGAt                                <  1:701476/49‑1 (MQ=255)               aGCTGCGATATTGCACAGTTTCTGCTTTGGGGTGATCTTCAATATGATc                               >  1:1917685/1‑49 (MQ=255)                gCTGCGATATTGCACAGTTTCTGCTTTGGGGTGATCTTCAATATGATCg                              <  1:682963/49‑1 (MQ=255)                    cGATATTGCACAGTTTCTGCTTTGGGGTGTTCTTCAATATGATCGTGc                           <  1:1097881/48‑1 (MQ=255)                    cGATATTGCACAGTTTCTGCTTTGGGGTGATCTTCAATATGATCGTGc                           <  1:1228265/48‑1 (MQ=255)                    cGATATTGCACAGTTTCTGCTTTGGGGTGATCTTCAATATGATCGTGCt                          >  1:178977/1‑49 (MQ=255)                     gATATTGCACAGTTTCTGCTTTGGGGTGATCTTCAATATGATCGTGCTg                         >  1:1228367/1‑49 (MQ=255)                     gATATTGCACAGTTTCTGCTTTGGGGTGATCTTCAATATGATCGTGCTg                         <  1:337359/49‑1 (MQ=255)                        atTGCACAGTTTCTGCTTTGGGGTGATCTTCAATATGATCGTGCTGgcg                      >  1:396776/1‑49 (MQ=255)                            cacaGTTTCTGCTTTGGGGTGATCTTCAATATGATCGTGCTGGCGCGt                   >  1:1087182/1‑48 (MQ=255)                             acaGTTTCTGCTTTGGGGTGATCTTCAATATGATCGTGCTGGCGCGTg                  >  1:948112/1‑48 (MQ=255)                             acaGTTTCTGCTTTGGGGTGATCTTCAATATGATCGTGCTGGCGCGTg                  >  1:495722/1‑48 (MQ=255)                                gTTTCTGCTTTGGGGTGATCTTCAATATGATCGTGCTGGCGCGTGAAgg              >  1:1208644/1‑49 (MQ=255)                                gTTTCTGCTTTGGGGTGATCTTCAATATGATCGTGCTGGCGCGTGAAgg              <  1:1183960/49‑1 (MQ=255)                                 tttCTGCTTTGGGGTGATCTTCAATATGATCGTGCTGGCGCGTGAAggg             >  1:1890431/1‑49 (MQ=255)                                   tCTGCTTTGGGGTGATCTTCAATATGATCGTGCTGGCGCGTGAAGGGc            <  1:2011269/48‑1 (MQ=255)                                    cTGCTTTGGGGTGATCTTCAATATGATCGTGCTGGCGCGTGAAGGGCTg          >  1:784950/1‑49 (MQ=255)                                         ttGGGGTGATCTTCAATATGATCGTGCTGGCGCGTGAAGGGCTGGATTc     >  1:1355338/1‑49 (MQ=255)                                          tGGGGTGATCTTCAATATGATCGTGCTGGCGCGTGAAGGGCTGGAtt      <  1:1499209/47‑1 (MQ=255)                                            gggTGATCTTCAATATGATCGTGCTGGCGCGTGAAGGGCTGGATTCGtt  >  1:202901/1‑49 (MQ=255)                                               |                                              CCTAATCGCTATAAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGCGATCTTCAATATGATCGTGCTGGCGCGTGAAGGGCTGGATTCGTT  >  NC_000913/3979723‑3979814

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑