Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,983,666	T→C	F237L (TTC→CTC)	aslB →	putative anaerobic sulfatase maturation enzyme AslB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,983,666	0	T	C	100.0%	62.8 / NA	17	F237L (TTC→CTC)	aslB	putative anaerobic sulfatase maturation enzyme AslB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/7);  total (10/7)

TGATATTCAGGGTGAGCTAACGGAATGGTCGGTGCGCCCCGAGGAGTTCGGTGAGTTTCTGGTGGCGATATTCGACCACTGGATCA  >  NC_000913/3983620‑3983705                                               |                                        tGATATTCAGGGTGAGCTAACGGAATGGTCGGTGCGCCCCGAGGAGCTc                                       >  1:42275/1‑49 (MQ=255)  gATATTCAGGGTGAGCTAACGGAATGGTCGGTGCGCCCCGAGGAGCTCg                                      >  1:170273/1‑49 (MQ=255)   atatTCAGGGTGAGCTAACGGAATGGTCGGTGCGCCCCGAGGAGCTCgg                                     >  1:1106607/1‑49 (MQ=255)   atatTCAGGGTGAGCTAACGGAATGGTCGGTGCGCCCCGAGGAGCTCg                                      <  1:1190889/48‑1 (MQ=255)         aGGGTGAGCTAACGGAATGGTCGGTGCGCCCCGAGGAGCTCGGTGAGtt                               >  1:387409/1‑49 (MQ=255)         aGGGTGAGCTAACGGAATGGTCGGTGCGCCCCGAGGAGCTCGGTGAGtt                               >  1:1066866/1‑49 (MQ=255)         aGGGTGAGCTAACGGAATGGTCGGTGCGCCCCGAGGAGCTCGGTGAGt                                <  1:1890983/48‑1 (MQ=255)          gggTGAGCTAACGGAATGGTCGGTGCGCCCCGAGGAGCTCGGTGAGttt                              <  1:1212639/49‑1 (MQ=255)           ggTGAGCTAACGGAATGGTCGGTGCGCCCCGAGGAGCTCGGTGAGTTTc                             <  1:23461/49‑1 (MQ=255)                  tAACGGAATGGTCGGTGCGCCCCGAGGAGCTCGGTGAGTTTCTGGTGGc                      >  1:1709334/1‑49 (MQ=255)                      ggAATGGTCGGTGCGCCCCGAGGAGCTCGGTGAGTTTCTGGTGGCGata                  <  1:1955080/49‑1 (MQ=255)                        aaTGGTCGGTGCGCCCCGAGGAGCTCGGTGAGTTTCTGGTGGCGATAtt                >  1:1837371/1‑49 (MQ=255)                         aTGGTCGGTGCGCCCCGAGGAGCTCGGTGAGTTTCTGGTGGCGATATTc               <  1:347821/49‑1 (MQ=255)                         aTGGTCGGTGCGCCCCGAGGAGCTCGGTGAGTTTCTGGTGGCGATATTc               <  1:1477982/49‑1 (MQ=255)                                gTGCGCCCCGAGGAGCTCGGTGAGTTTCTGGTGGCGATATTCGACCACt        >  1:1139442/1‑49 (MQ=255)                                    gcCCCGAGGAGCTCGGTGAGTTTCTGGTGGCGATATTCGACCACTGGAt    >  1:1990436/1‑49 (MQ=255)                                       ccGAGGAGCTCGGTGAGTTTCTGGTGGCGATATTCGACCACTGGATCa  >  1:677774/1‑48 (MQ=255)                                               |                                        TGATATTCAGGGTGAGCTAACGGAATGGTCGGTGCGCCCCGAGGAGTTCGGTGAGTTTCTGGTGGCGATATTCGACCACTGGATCA  >  NC_000913/3983620‑3983705

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑