Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,093,223	A→G	V72V (GTT→GTC)	rhaM ←	L‑rhamnose mutarotase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,093,223	0	A	G	100.0%	100.9 / NA	26	V72V (GTT→GTC)	rhaM	L‑rhamnose mutarotase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (8/18);  total (8/18)

TTAGCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAAACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGATTCAAT  >  NC_000913/4093179‑4093267                                             |                                             ttAGCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACAt                                          >  1:817399/1‑49 (MQ=255)    gCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAg                                       <  1:1349982/49‑1 (MQ=255)     cGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGt                                      >  1:308487/1‑49 (MQ=255)     cGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGt                                      <  1:47738/49‑1 (MQ=255)      gggCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTg                                     >  1:1921535/1‑49 (MQ=255)         cATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTg                                  <  1:1494424/49‑1 (MQ=255)         cATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTg                                  <  1:1656306/49‑1 (MQ=255)         cATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTg                                  <  1:815324/49‑1 (MQ=255)                tCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCg                           >  1:457786/1‑49 (MQ=255)                    tCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCAtt                       >  1:1399697/1‑49 (MQ=255)                         tatTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGc                  <  1:806143/49‑1 (MQ=255)                         tatTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGc                  <  1:481882/49‑1 (MQ=255)                             tCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGtt               <  1:558658/48‑1 (MQ=255)                              ccaccaACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGttc              <  1:159466/48‑1 (MQ=255)                              ccaccaACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGttc              <  1:510103/48‑1 (MQ=255)                               caccaACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGttctt            >  1:835477/1‑49 (MQ=255)                                accaACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGttctt            >  1:548545/1‑48 (MQ=255)                                 ccaACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCa          <  1:1055139/49‑1 (MQ=255)                                  caACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAg         <  1:1790088/49‑1 (MQ=255)                                   aaCGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGttctt            >  1:1603363/1‑45 (MQ=255)                                     cGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGAtt      <  1:1980887/49‑1 (MQ=255)                                      gTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGATTc     <  1:321687/49‑1 (MQ=255)                                      gTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGATTc     <  1:1920312/49‑1 (MQ=255)                                      gTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGATTc     <  1:620516/49‑1 (MQ=255)                                       ttGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGATTCa    <  1:379381/49‑1 (MQ=255)                                          gCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGATTCAAt  <  1:1906781/48‑1 (MQ=255)                                             |                                             TTAGCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAAACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGATTCAAT  >  NC_000913/4093179‑4093267

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑