Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	988,499	G→A	P163S (CCA→TCA)	asnS ←	asparagine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	988,499	0	G	A	100.0%	70.1 / NA	19	P163S (CCA→TCA)	asnS	asparagine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/10);  total (9/10)

GAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGGCGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGC  >  NC_000913/988455‑988542                                             |                                            gAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGtt                                         >  1:82675/1‑49 (MQ=255)  aaCATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTg                                        <  1:672528/49‑1 (MQ=255)  aaCATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTg                                        <  1:1773675/49‑1 (MQ=255)       ttCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAAcc                                   >  1:937384/1‑49 (MQ=255)        tCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAAcc                                   <  1:654595/48‑1 (MQ=255)          gCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAAcc                                   >  1:1647676/1‑46 (MQ=255)           ccTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCag                                >  1:2064955/1‑48 (MQ=255)               cACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCagaaga                            <  1:2024876/48‑1 (MQ=255)               cACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAgaagaa                           <  1:323103/49‑1 (MQ=255)                aCCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAAt                          <  1:1026064/49‑1 (MQ=255)                aCCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAAt                          >  1:1115458/1‑49 (MQ=255)                      ggTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGc                    >  1:1925025/1‑49 (MQ=255)                      ggTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGc                    <  1:1179285/49‑1 (MQ=255)                      ggTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGc                    >  1:1003088/1‑49 (MQ=255)                        tATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTc                  <  1:1400735/49‑1 (MQ=255)                                 cGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAaaga          <  1:410879/48‑1 (MQ=255)                                   gTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGc       >  1:657612/1‑49 (MQ=255)                                     aaTCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGa     >  1:1250114/1‑49 (MQ=255)                                         aGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGc  <  1:1814476/48‑1 (MQ=255)                                             |                                            GAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGGCGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGC  >  NC_000913/988455‑988542

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑