Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,154,362	C→T	A84V (GCC→GTC)	mltG →	endolytic murein transglycosylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,154,362	0	C	T	100.0%	96.1 / NA	24	A84V (GCC→GTC)	mltG	endolytic murein transglycosylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/13);  total (11/13)

AATGGCTGCTGCGTATCGAACCGGATCTTTCTCACTTTAAAGCCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCGAG  >  NC_000913/1154320‑1154402                                           |                                         aaTGGCTGCTGCGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGa                                     <  1:2000271/48‑1 (MQ=255)  aTGGCTGCTGCGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACt                                   <  1:1844945/49‑1 (MQ=255)    ggCTGCTGCGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTa                                 >  1:1070015/1‑49 (MQ=255)     gctgctGCGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTa                                 <  1:456696/48‑1 (MQ=255)      ctgctgCGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTAcc                               <  1:484819/49‑1 (MQ=255)      ctgctgCGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTAcc                               <  1:366428/49‑1 (MQ=255)       tgctgcGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCg                              >  1:1355442/1‑49 (MQ=255)       tgctgcGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCg                              >  1:1754863/1‑49 (MQ=255)          tgcGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCtt                           >  1:1972773/1‑49 (MQ=255)            cGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTa                         <  1:839539/49‑1 (MQ=255)            cGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTa                         >  1:798698/1‑49 (MQ=255)              tATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTaca                       <  1:1153356/49‑1 (MQ=255)              tATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTaca                       >  1:54131/1‑49 (MQ=255)                  gAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCg                    >  1:1686977/1‑48 (MQ=255)                   aaCCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCg                    >  1:49489/1‑47 (MQ=255)                    aCCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCGCAg                 <  1:869333/49‑1 (MQ=255)                      cGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGAt               <  1:667435/49‑1 (MQ=255)                       ggATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGAt               >  1:1920080/1‑48 (MQ=255)                        gATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGa             >  1:18286/1‑49 (MQ=255)                              tctcACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGAcc           <  1:819722/45‑1 (MQ=255)                              tctcACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCg          <  1:584070/46‑1 (MQ=255)                              tctcACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCGt         <  1:1345184/47‑1 (MQ=255)                                tcACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCGTgcgc     >  1:644750/1‑49 (MQ=255)                                   cTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCgag  <  1:1949432/49‑1 (MQ=255)                                           |                                         AATGGCTGCTGCGTATCGAACCGGATCTTTCTCACTTTAAAGCCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCGAG  >  NC_000913/1154320‑1154402

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑