Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,240,439	C→T	V60V (GTC→GTT)  D545N (GAC→AAC)	ymgM → cvrA ←	protein YmgM putative K(+):H(+) antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,240,439	0	C	T	100.0%	55.5 / NA	15	V60V (GTC→GTT)  D545N (GAC→AAC)	ymgM cvrA	protein YmgM putative K(+):H(+) antiporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/11);  total (4/11)

CTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTC  >  NC_000913/1240398‑1240481                                          |                                           ctcGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTTACCGAc                                      <  1:1489713/48‑1 (MQ=255)    ggCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTTACCGACa                                     <  1:993854/46‑1 (MQ=255)    ggCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTTACCGACAAcc                                  >  1:351423/1‑49 (MQ=255)     gCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTTACCGACAACCg                                 <  1:484653/49‑1 (MQ=255)        aCCGTCCAGATCATCCCGGCAAACTCTACCTGGTTACCGACAACCGGTg                              >  1:1110664/1‑49 (MQ=255)           gTCCAGATCATCCCGGCAAACTCTACCTGGTTACCGACAACCGGTGCcg                           >  1:1159641/1‑49 (MQ=255)            tCCAGATCATCCCGGCAAACTCTACCTGGTTACCGACAACCGGTGCcgc                          <  1:1281552/49‑1 (MQ=255)             ccAGATCATCCCGGCAAACTCTACCTGGTTACCGACAACCGGTGCcgcg                         >  1:1292050/1‑49 (MQ=255)                gATCATCCCGGCAAACTCTACCTGGTTACCGACAACCGGTGCCGCGCCt                      <  1:1010614/49‑1 (MQ=255)                gATCATCCCGGCAAACTCTACCTGGTTACCGACAACCGGTGCCGCGCCt                      <  1:641859/49‑1 (MQ=255)                gATCATCCCGGCAAACTCTACCTGGTTACCGACAACCGGTGCCGCGCCt                      <  1:951193/49‑1 (MQ=255)                        cGGCAAACTCTACCTGGTTACCGACAACCGGTGCCGCGCCTAACAActg              <  1:888415/49‑1 (MQ=255)                               ctctACCTGGTTACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACa       <  1:1685945/49‑1 (MQ=255)                                    ccTGGTTACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTc  <  1:1663817/49‑1 (MQ=255)                                    ccTGGTTACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTc  <  1:451862/49‑1 (MQ=255)                                          |                                           CTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTC  >  NC_000913/1240398‑1240481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑