Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,270,811	C→T	V347M (GTG→ATG)	chaA ←	Na(+)/K(+):H(+) antiporter ChaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,270,811	0	C	T	100.0%	110.6 / NA	28	V347M (GTG→ATG)	chaA	Na(+)/K(+):H(+) antiporter ChaA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/16);  total (12/16)

CAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAG  >  NC_000913/1270767‑1270855                                             |                                             caAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGt                                            >  1:601180/1‑47 (MQ=255) caAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGt                                            >  1:434971/1‑47 (MQ=255)   aaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAg                                         >  1:1940933/1‑48 (MQ=255)     tAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTAc                                      <  1:1541013/49‑1 (MQ=255)      aGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACg                                     >  1:657978/1‑49 (MQ=255)      aGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACg                                     >  1:295229/1‑49 (MQ=255)          ggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCg                                 >  1:236828/1‑49 (MQ=255)           gcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCgg                                >  1:1398994/1‑49 (MQ=255)            cAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGt                               <  1:950635/49‑1 (MQ=255)             aaaCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg                              <  1:644203/49‑1 (MQ=255)             aaaCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg                              <  1:222260/49‑1 (MQ=255)              aaCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTgg                             <  1:5288/49‑1 (MQ=255)               aCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGga                            <  1:956552/49‑1 (MQ=255)                   tGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                        <  1:1185036/49‑1 (MQ=255)                   tGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                        <  1:1016751/49‑1 (MQ=255)                   tGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                        <  1:100503/49‑1 (MQ=255)                     ccAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAgg                       <  1:1703312/48‑1 (MQ=255)                       aGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGgaga                    <  1:1958430/49‑1 (MQ=255)                            cgccgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATg                <  1:1188311/48‑1 (MQ=255)                             gctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATgg               >  1:750378/1‑48 (MQ=255)                                 cgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAg          >  1:852611/1‑49 (MQ=255)                                  gcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAg          >  1:2051079/1‑48 (MQ=255)                                   ccATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCa        <  1:1121504/49‑1 (MQ=255)                                    cATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCAc       <  1:1628580/49‑1 (MQ=255)                                    cATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCAc       >  1:158385/1‑49 (MQ=255)                                     aTTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACt      <  1:370463/49‑1 (MQ=255)                                         aGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTa     >  1:1417822/1‑46 (MQ=255)                                         aGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAag  <  1:530315/49‑1 (MQ=255)                                             |                                             CAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAG  >  NC_000913/1270767‑1270855

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑