Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,382,555	C→T	S203S (AGC→AGT)	ompG →	outer membrane porin G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,382,555	0	C	T	100.0%	91.5 / NA	23	S203S (AGC→AGT)	ompG	outer membrane porin G
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/14);  total (9/14)

GAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGCCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTA  >  NC_000913/1382512‑1382596                                            |                                          gAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCaa                                      <  1:415735/49‑1 (MQ=255)    tGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATaa                                   >  1:4924/1‑49 (MQ=255)    tGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATaa                                   >  1:1995138/1‑49 (MQ=255)     gAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAAc                                  >  1:694798/1‑49 (MQ=255)      aaCTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACg                                 <  1:722122/49‑1 (MQ=255)       aCTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACgg                                >  1:1277304/1‑49 (MQ=255)        cTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGt                               >  1:1802126/1‑49 (MQ=255)           ttatCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTg                              <  1:1824982/47‑1 (MQ=255)             atCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGtt                          >  1:358416/1‑49 (MQ=255)              tctcGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGtt                          <  1:1257528/48‑1 (MQ=255)                 cGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTc                       >  1:634164/1‑48 (MQ=255)                  gAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGtttt                        <  1:1297533/46‑1 (MQ=255)                  gAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGttt                         <  1:1595127/45‑1 (MQ=255)                  gAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGttt                         <  1:672035/45‑1 (MQ=255)                  gAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTcc                      <  1:681096/48‑1 (MQ=255)                      gcgGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCa                 <  1:1708683/49‑1 (MQ=255)                           ttCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGaaa            >  1:539387/1‑49 (MQ=255)                              aaTATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAAtt          <  1:760575/48‑1 (MQ=255)                              aaTATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTc         >  1:228447/1‑49 (MQ=255)                                  tGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTcgcg      <  1:1691208/48‑1 (MQ=255)                                    gacgacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCt   <  1:1626107/49‑1 (MQ=255)                                     acgacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTa  <  1:1604651/49‑1 (MQ=255)                                     acgacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTa  <  1:765630/49‑1 (MQ=255)                                            |                                          GAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGCCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTA  >  NC_000913/1382512‑1382596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑