Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	119,028	T→C	V99A (GTC→GCC)	ampD →	1,6‑anhydro‑N‑acetylmuramoyl‑L‑alanine amidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	119,028	0	T	C	100.0%	81.4 / NA	22	V99A (GTC→GCC)	ampD	1,6‑anhydro‑N‑acetylmuramoyl‑L‑alanine amidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/8);  total (14/8)

CCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGTCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTATTG  >  NC_000913/118987‑119072                                          |                                             ccAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAg                                       >  1:1513746/1‑49 (MQ=255)  cAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAg                                       <  1:514173/48‑1 (MQ=255)  cAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGt                                      <  1:1983767/49‑1 (MQ=255)     tATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATc                                   <  1:406744/49‑1 (MQ=255)       tGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAg                                 >  1:1206088/1‑49 (MQ=255)        gTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAgg                                >  1:895945/1‑49 (MQ=255)         ttCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAggg                               <  1:150566/49‑1 (MQ=255)            cTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGgc                             <  1:1767745/48‑1 (MQ=255)            cTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGgc                             <  1:1870738/48‑1 (MQ=255)               tCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGcgcg                          >  1:134395/1‑48 (MQ=255)                cGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGaa                        >  1:1539361/1‑49 (MQ=255)                cGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGaa                        >  1:1148515/1‑49 (MQ=255)                      aCGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTg                   >  1:1430653/1‑48 (MQ=255)                          gcatggcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAAt               >  1:372103/1‑48 (MQ=255)                          gcatggcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAAt               >  1:42128/1‑48 (MQ=255)                              ggcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAttt          >  1:570367/1‑49 (MQ=255)                               gcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAtttt         >  1:543911/1‑49 (MQ=255)                                catgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAttttt        >  1:993579/1‑49 (MQ=255)                                 atgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTc       <  1:180959/49‑1 (MQ=255)                                  tgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCt      <  1:830698/49‑1 (MQ=255)                                     gggAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTAtt   >  1:1585641/1‑49 (MQ=255)                                      ggAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTATTg  >  1:825305/1‑49 (MQ=255)                                          |                                             CCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGTCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTATTG  >  NC_000913/118987‑119072

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑