Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,562,085	C→T	A133T (GCA→ACA)	ddpA ←	putative D,D‑dipeptide ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,562,085	0	C	T	100.0%	146.1 / NA	37	A133T (GCA→ACA)	ddpA	putative D,D‑dipeptide ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/18);  total (19/18)

CGTATGTTCGTCGGGAGCATCAATCTTTAAATCTTTGGGAAATGCTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTCAAAAGAAAG  >  NC_000913/1562041‑1562130                                             |                                              cGTATGTTCGTCGGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTct                                           <  1:265459/49‑1 (MQ=255) cGTATGTTCGTCGGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTct                                           <  1:207652/49‑1 (MQ=255)   tATGTTCGTCGGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTctgc                                         <  1:1010871/49‑1 (MQ=255)   tATGTTCGTCGGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTctgc                                         >  1:1418301/1‑49 (MQ=255)    aTGTTCGTCGGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTctgct                                        >  1:782616/1‑49 (MQ=255)     tGTTCGTCGGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTctgctg                                       <  1:565637/49‑1 (MQ=255)       ttCGTCGGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTgg                                      <  1:493533/48‑1 (MQ=255)       ttCGTCGGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGc                                     <  1:1395999/49‑1 (MQ=255)          gtcgGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGcccc                                  <  1:802358/49‑1 (MQ=255)          gtcgGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGcccc                                  >  1:1606403/1‑49 (MQ=255)          gtcgGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGcccc                                  >  1:2005754/1‑49 (MQ=255)           tcgGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCt                                 >  1:1765151/1‑49 (MQ=255)             gggAGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTg                                <  1:924009/48‑1 (MQ=255)                  cATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGAt                          >  1:2027854/1‑49 (MQ=255)                   aTCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGAtt                         >  1:1779563/1‑49 (MQ=255)                   aTCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGAtt                         <  1:907133/49‑1 (MQ=255)                      aaTCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGAttttt                      <  1:1280430/49‑1 (MQ=255)                      aaTCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGAtttt                       >  1:1246034/1‑48 (MQ=255)                        tCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTtag                    >  1:1074857/1‑49 (MQ=255)                         cTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTtag                    >  1:2016571/1‑48 (MQ=255)                         cTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTtag                    >  1:1972818/1‑48 (MQ=255)                           ttAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTtagtag                 >  1:248323/1‑49 (MQ=255)                            tAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTtagtag                 <  1:407153/48‑1 (MQ=255)                            tAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTtagt                   <  1:78145/46‑1 (MQ=255)                            tAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTtagt                   <  1:60101/46‑1 (MQ=255)                              aaTCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGcc               <  1:820002/48‑1 (MQ=255)                                  tttGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTc           >  1:1565539/1‑48 (MQ=255)                                   ttGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTCa          >  1:27407/1‑48 (MQ=255)                                   ttGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTCa          >  1:1173405/1‑48 (MQ=255)                                    tGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTCaa         >  1:523415/1‑48 (MQ=255)                                    tGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTCaa         >  1:279644/1‑48 (MQ=255)                                    tGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTCa          <  1:486804/47‑1 (MQ=255)                                    tGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTCa          <  1:1271234/47‑1 (MQ=255)                                      ggAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTCaa         >  1:2013641/1‑46 (MQ=255)                                      ggAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTCAaaag      <  1:1648534/49‑1 (MQ=255)                                       gAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTCAaaaga     <  1:608291/49‑1 (MQ=255)                                          aTGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTCAaaagaaag  >  1:548919/1‑49 (MQ=255)                                             |                                              CGTATGTTCGTCGGGAGCATCAATCTTTAAATCTTTGGGAAATGCTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTCAAAAGAAAG  >  NC_000913/1562041‑1562130

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑