Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,662,872	C→T	R773W (CGG→TGG)	ynfF →	putative selenate reductase YnfF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,662,872	0	C	T	100.0%	102.9 / NA	26	R773W (CGG→TGG)	ynfF	putative selenate reductase YnfF
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/18);  total (8/18)

GCCATCGGGCAAGGTGCGTGGCTTAAAGCGGATATGTTTGGTGACCGGGTCGATCACGGCGGCAGTATCAATATTCTGACCTCTCACC  >  NC_000913/1662827‑1662914                                              |                                           gCCATCGGGCAAGGTGCGTGGCTTAAAGCGGATATGTTTGGTGACTggg                                         >  1:1798228/1‑49 (MQ=255)   cATCGGGCAAGGTGCGTGGCTTAAAGCGGATATGTTTGGTGACTGGGTc                                       <  1:1406990/49‑1 (MQ=255)    aTCGGGCAAGGTGCGTGGCTTAAAGCGGATATGTTTGGTGACTGGGTCg                                      <  1:540670/49‑1 (MQ=255)     tCGGGCAAGGTGCGTGGCTTAAAGCGGATATGTTTGGTGACTGGGTCGa                                     >  1:45656/1‑49 (MQ=255)       gggCAAGGTGCGTGGCTTAAAGCGGATATGTTTGGTGACTGGGTCGAt                                    <  1:1760927/48‑1 (MQ=255)             ggTGCGTGGCTTAAAGCGGATATGTTTGGTGACTGGGTCGATCAcggcg                             <  1:1764102/49‑1 (MQ=255)               tGCGTGGCTTAAAGCGGATATGTTTGGTGACTGGGTCGATCAcggcgg                            >  1:1716746/1‑48 (MQ=255)               tGCGTGGCTTAAAGCGGATATGTTTGGTGACTGGGTCGATCACggcggc                           <  1:805478/49‑1 (MQ=255)               tGCGTGGCTTAAAGCGGATATGTTTGGTGACTGGGTCGATCACggcggc                           <  1:1354564/49‑1 (MQ=255)                 cGTGGCTTAAAGCGGATATGTTTGGTGACTGGGTCGATCACGGCGGCa                          <  1:1366720/48‑1 (MQ=255)                 cGTGGCTTAAAGCGGATATGTTTGGTGACTGGGTCGATCACGGCGGCa                          <  1:703471/48‑1 (MQ=255)                 cGTGGCTTAAAGCGGATATGTTTGGTGACTGGGTCGATCACGGCGGCAg                         >  1:1431521/1‑49 (MQ=255)                  gTGGCTTAAAGCGGATATGTTTGGTGACTGGGTCGATCACGGCGGCAg                         >  1:540721/1‑48 (MQ=255)                  gTGGCTTAAAGCGGATATGTTTGGTGACTGGGTCGATCACGGCGGCAg                         >  1:625493/1‑48 (MQ=255)                  gTGGCTTAAAGCGGATATGTTTGGTGACTGGGTCGATCACGGCGGCAGt                        <  1:1514401/49‑1 (MQ=255)                  gTGGCTTAAAGCGGATATGTTTGGTGACTGGGTCGATCACGGCGGCAGt                        <  1:1189324/49‑1 (MQ=255)                      cTTAAAGCGGATATGTTTGGTGACTGGGTCGATCACGGCGGCAGTATCa                    <  1:45558/49‑1 (MQ=255)                           aGCGGATATGTTTGGTGACTGGGTCGATCACGGCGGCAGTATCAATAtt               <  1:1382699/49‑1 (MQ=255)                             cGGATATGTTTGGTGACTGGGTCGATCACGGCGGCAGTATCAATATTCt             <  1:1243067/49‑1 (MQ=255)                                atatGTTTGGTGACTGGGTCGATCACGGCGGCAGTATCAATATTCTGAc          >  1:114041/1‑49 (MQ=255)                                  atGTTTGGTGACTGGGTCGATCACGGCGGCAGTATCAATATTCTGACct        >  1:486602/1‑49 (MQ=255)                                   tGTTTGGTGACTGGGTCGATCACGGCGGCAGTATCAATATTCTGACct        <  1:1871541/48‑1 (MQ=255)                                     tttGGTGACTGGGTCGATCACGGCGGCAGTATCAATATTCTGACCtctc     <  1:943597/49‑1 (MQ=255)                                        ggTGACTGGGTCGATCACGGCGGCAGTATCAATATTCTGACCTCTCAc   <  1:937116/48‑1 (MQ=255)                                         gTGACTGGGTCGATCACGGCGGCAGTATCAATATTCTGACCTCTCAcc  <  1:792550/48‑1 (MQ=255)                                         gTGACTGGGTCGATCACGGCGGCAGTATCAATATTCTGACCTCTCAcc  <  1:1141098/48‑1 (MQ=255)                                              |                                           GCCATCGGGCAAGGTGCGTGGCTTAAAGCGGATATGTTTGGTGACCGGGTCGATCACGGCGGCAGTATCAATATTCTGACCTCTCACC  >  NC_000913/1662827‑1662914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑