Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,697,216	T→C	intergenic (‑164/+57)	uidR ← / ← hdhA	DNA‑binding transcriptional repressor UidR/7‑alpha‑hydroxysteroid dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,697,216	0	T	C	100.0%	141.4 / NA	36	intergenic (‑164/+57)	uidR/hdhA	DNA‑binding transcriptional repressor UidR/7‑alpha‑hydroxysteroid dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/19);  total (17/19)

GTTAACCAATAGCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGT  >  NC_000913/1697170‑1697261                                               |                                              gTTAACCAATAGCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCtg                                             <  1:1995455/49‑1 (MQ=255)  ttAACCAATAGCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCtga                                            >  1:326472/1‑49 (MQ=255)     aCCAATAGCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCtgatg                                          <  1:931418/48‑1 (MQ=255)     aCCAATAGCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCtgatg                                          <  1:925588/48‑1 (MQ=255)     aCCAATAGCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCtgat                                           >  1:271171/1‑47 (MQ=255)      ccAATAGCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAg                                        >  1:561634/1‑49 (MQ=255)       cAATAGCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGt                                       >  1:395062/1‑49 (MQ=255)       cAATAGCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGt                                       >  1:1879279/1‑49 (MQ=255)        aaTAGCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGtt                                      >  1:987832/1‑49 (MQ=255)          tAGCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTa                                    <  1:1226383/49‑1 (MQ=255)          tAGCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTa                                    >  1:1701623/1‑49 (MQ=255)            gCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTAt                                   <  1:1376465/48‑1 (MQ=255)               gTGATTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCg                               <  1:1569418/49‑1 (MQ=255)               gTGATTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCg                               <  1:1583506/49‑1 (MQ=255)                   tttGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTaa                           <  1:1889654/49‑1 (MQ=255)                   tttGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTaa                           <  1:2026758/49‑1 (MQ=255)                   tttGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTa                            >  1:106274/1‑48 (MQ=255)                      ggCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTAaac                         >  1:161644/1‑48 (MQ=255)                      ggCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTAaac                         <  1:2044238/48‑1 (MQ=255)                      ggCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTAaac                         <  1:1155868/48‑1 (MQ=255)                        cTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTAaacaac                      <  1:360662/49‑1 (MQ=255)                          gCCAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTAAACAacac                    >  1:1111645/1‑49 (MQ=255)                          gCCAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTAAACAacac                    >  1:1266636/1‑49 (MQ=255)                           ccAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTAAacaaca                     <  1:1741325/47‑1 (MQ=255)                           ccAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTAAACAAcacg                   >  1:16426/1‑49 (MQ=255)                              gTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTAAACAAcacgcac                >  1:663511/1‑49 (MQ=255)                                   ttAGCAGAGACGCTGATGAGTTTATCCCGGTAAACAACACGCACGgttg           >  1:1911300/1‑49 (MQ=255)                                   ttAGCAGAGACGCTGATGAGTTTATCCCGGTAAACAACACGCACGgttg           >  1:271977/1‑49 (MQ=255)                                     aGCAGAGACGCTGATGAGTTTATCCCGGTAAACAACACGCACGgttgt          <  1:1498636/48‑1 (MQ=255)                                      gCAGAGACGCTGATGAGTTTATCCCGGTAAACAACACGCACGgttgtt         <  1:1687092/48‑1 (MQ=255)                                       cAGAGACGCTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTa       >  1:281105/1‑49 (MQ=255)                                        agagACGCTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTAc      <  1:1348158/49‑1 (MQ=255)                                        agagACGCTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTAc      <  1:1581874/49‑1 (MQ=255)                                         gagaCGCTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTAcc     >  1:328122/1‑49 (MQ=255)                                          agaCGCTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCg    <  1:144262/49‑1 (MQ=255)                                            aCGCTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGt  <  1:591146/49‑1 (MQ=255)                                               |                                              GTTAACCAATAGCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGT  >  NC_000913/1697170‑1697261

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑