Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,741,062	T→C	C384C (TGT→TGC)	ydhC →	putative transporter YdhC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,741,062	0	T	C	100.0%	83.4 / NA	22	C384C (TGT→TGC)	ydhC	putative transporter YdhC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/9);  total (13/9)

CAACAGTAGTGCTGGTCGCGCTGGGTTACATGATGCAACGTTGTGAAGAAGTTGGCTGCCAGAATCATGGCAATGCCGAAGTCGCTCAT  >  NC_000913/1741019‑1741107                                            |                                              cAACAGTAGTGCTGGTCGCGCTGGGTTACATGATGCAACGTTGCgaag                                           >  1:906948/1‑48 (MQ=255)  aaCAGTAGTGCTGGTCGCGCTGGGTTACATGATGCAACGTTGCgaag                                           >  1:1424498/1‑47 (MQ=255)     agtagtGCTGGTCGCGCTGGGTTACATGATGCAACGTTGCGAAGAAGtt                                      >  1:659357/1‑49 (MQ=255)       tagtGCTGGTCGCGCTGGGTTACATGATGCAACGTTGCGAAGAAGTTgg                                    >  1:2016262/1‑49 (MQ=255)          tGCTGGTCGCGCTGGGTTACATGATGCAACGTTGCGAAGAAGTTGGCTg                                 >  1:694283/1‑49 (MQ=255)              ggTCGCGCTGGGTTACATGATGCAACGTTGCGAAGAAGTTGGCTGc                                >  1:1393230/1‑46 (MQ=255)                tCGCGCTGGGTTACATGATGCAACGTTGCGAAGAAGTTGGCTGCCAGaa                           >  1:2052898/1‑49 (MQ=255)                  gcgcTGGGTTACATGATGCAACGTTGCGAAGAAGTTGGCTGCCAGAAt                          >  1:665470/1‑48 (MQ=255)                  gcgcTGGGTTACATGATGCAACGTTGCGAAGAAGTTGGCTGCCAGAAt                          >  1:216569/1‑48 (MQ=255)                    gcTGGGTTACATGATGCAACGTTGCGAAGAAGTTGGCTGCCAGAATCAt                       >  1:262944/1‑49 (MQ=255)                      tGGGTTACATGATGCAACGTTGCGAAGAAGTTGGCTGCCAGAATCATgg                     >  1:947827/1‑49 (MQ=255)                      tGGGTTACATGATGCAACGTTGCGAAGAAGTTGGCTGCCAGAATCATgg                     >  1:470460/1‑49 (MQ=255)                        ggTTACATGATGCAACGTTGCGAAGAAGTTGGCTGCCAGAATCATGGc                    <  1:1492862/48‑1 (MQ=255)                        ggTTACATGATGCAACGTTGCGAAGAAGTTGGCTGCCAGAATCATGGCa                   <  1:1924637/49‑1 (MQ=255)                             cATGATGCAACGTTGCGAAGAAGTTGGCTGCCAGAATCATGGCAATGcc              <  1:738182/49‑1 (MQ=255)                                 atgCAACGTTGCGAAGAAGTTGGCTGCCAGAATCATGGCAATGCCGaa           <  1:1299061/48‑1 (MQ=255)                                 atgCAACGTTGCGAAGAAGTTGGCTGCCAGAATCATGGCAATGCCGAAg          <  1:1229619/49‑1 (MQ=255)                                    cAACGTTGCGAAGAAGTTGGCTGCCAGAATCATGGCAATGCCGAAGTc        <  1:854752/48‑1 (MQ=255)                                    cAACGTTGCGAAGAAGTTGGCTGCCAGAATCATGGCAATGCCGAAGTCg       >  1:1589968/1‑49 (MQ=255)                                       cGTTGCGAAGAAGTTGGCTGCCAGAATCATGGCAATGCCGAAGTCGCTc    <  1:1101911/49‑1 (MQ=255)                                         ttGCGAAGAAGTTGGCTGCCAGAATCATGGCAATGCCGAAGTCGCTCAt  <  1:225752/49‑1 (MQ=255)                                         ttGCGAAGAAGTTGGCTGCCAGAATCATGGCAATGCCGAAGTCGCTCAt  <  1:1243524/49‑1 (MQ=255)                                            |                                              CAACAGTAGTGCTGGTCGCGCTGGGTTACATGATGCAACGTTGTGAAGAAGTTGGCTGCCAGAATCATGGCAATGCCGAAGTCGCTCAT  >  NC_000913/1741019‑1741107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑