Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,742,755	G→C	P163R (CCG→CGG)	ribC ←	riboflavin synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,742,755	0	G	C	100.0%	90.3 / NA	24	P163R (CCG→CGG)	ribC	riboflavin synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (14/10);  total (14/10)

CGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCGGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACG  >  NC_000913/1742711‑1742799                                             |                                             cgcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGaa                                           <  1:1614333/48‑1 (MQ=255)    cAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTaa                                       <  1:1799192/49‑1 (MQ=255)     aaGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTaaa                                      <  1:284311/49‑1 (MQ=255)     aaGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTaaa                                      <  1:1472687/49‑1 (MQ=255)     aaGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTaaa                                      >  1:1569632/1‑49 (MQ=255)        ttttttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATgg                                   <  1:1219595/49‑1 (MQ=255)        ttttttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATg                                    >  1:257571/1‑48 (MQ=255)           tttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACg                                >  1:627285/1‑49 (MQ=255)                 cccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaaa                            <  1:184732/47‑1 (MQ=255)                 cccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaaa                            <  1:550929/47‑1 (MQ=255)                  ccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAc                         <  1:183796/49‑1 (MQ=255)                    aaGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAcgc                       >  1:494113/1‑49 (MQ=255)                        gTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTg                   <  1:2101555/49‑1 (MQ=255)                          cGTGCGTTCCAGTGTTTCTCGAATTAAATGGACGCAAAAACGCGTTGGc                 >  1:1395124/1‑49 (MQ=39)                           gTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCg                >  1:650980/1‑49 (MQ=255)                              cGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGa             >  1:137303/1‑49 (MQ=255)                               gTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGAc            >  1:1175209/1‑49 (MQ=255)                               gTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGAc            >  1:90865/1‑49 (MQ=255)                                   cAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTc         <  1:735041/48‑1 (MQ=255)                                     gtgtTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGcc      >  1:314937/1‑49 (MQ=255)                                       gtTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGa    >  1:415253/1‑49 (MQ=255)                                       gtTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGa    >  1:56866/1‑49 (MQ=255)                                         ttCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACg  >  1:698033/1‑49 (MQ=255)                                         ttCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACg  >  1:779623/1‑49 (MQ=255)                                             |                                             CGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCGGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACG  >  NC_000913/1742711‑1742799

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑