Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,776,533	G→A	G316D (GGC→GAC)	ydiF →	putative acetate‑CoA transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,776,533	0	G	A	100.0%	133.8 / NA	34	G316D (GGC→GAC)	ydiF	putative acetate‑CoA transferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/17);  total (17/17)

GGCGCGGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGGCCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTCTGAC  >  NC_000913/1776490‑1776578                                            |                                              ggCGCGGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGACCTgg                                          >  1:47894/1‑49 (MQ=255)  gcgcGGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGACCTGGt                                         <  1:1964791/49‑1 (MQ=255)   cgcgGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGACCTGGTc                                        <  1:1790837/49‑1 (MQ=255)   cgcgGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGACCTGGTc                                        >  1:1642278/1‑49 (MQ=255)    gcgGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGACCTGGTCg                                       >  1:1011595/1‑49 (MQ=255)    gcgGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGACCTGGTCg                                       >  1:786246/1‑49 (MQ=255)    gcgGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGACCTGGTCg                                       <  1:1350616/49‑1 (MQ=255)      ggTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGACCTGGTCGcc                                     >  1:1019284/1‑49 (MQ=255)       gTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGACCTGGTCGccc                                    >  1:827040/1‑49 (MQ=255)       gTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGACCTGGTCGccc                                    <  1:439035/49‑1 (MQ=255)       gTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGACCTGGTCGccc                                    <  1:344076/49‑1 (MQ=255)        tGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGACCTGGTCGCCCg                                   <  1:2061762/49‑1 (MQ=255)          gggAATGTCGGCGTCGGTATTGCTGACGGCATTGACCTGGTCGccc                                    >  1:1167218/1‑46 (MQ=255)            gAATGTCGGCGTCGGTATTGCTGACGGCATTGACCTGGTCGCCCgag                                 >  1:44001/1‑47 (MQ=255)             aaTGTCGGCGTCGGTATTGCTGACGGCATTGACCTGGTCGCCCGagaag                              <  1:967484/49‑1 (MQ=255)               tGTCGGCGTCGGTATTGCTGACGGCATTGACCTGGTCGCCCGAgaagaa                            <  1:498324/49‑1 (MQ=255)                 tCGGCGTCGGTATTGCTGACGGCATTGACCTGGTCGCCCGAGAAGAAgg                          >  1:1560555/1‑49 (MQ=255)                 tCGGCGTCGGTATTGCTGACGGCATTGACCTGGTCGCCCGAGAAGAAgg                          >  1:294996/1‑49 (MQ=255)                   ggCGTCGGTATTGCTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGt                         <  1:1190925/48‑1 (MQ=255)                      gTCGGTATTGCTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTtgtg                     >  1:405606/1‑49 (MQ=255)                      gTCGGTATTGCTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTtgtg                     >  1:1360345/1‑49 (MQ=255)                        cGGTATTGCTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCt                   >  1:1578284/1‑49 (MQ=255)                         ggTATTGCTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCt                   >  1:2058282/1‑48 (MQ=255)                           tATTGCTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCtgat                <  1:1263010/49‑1 (MQ=255)                            aTTGCTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCtgat                >  1:1790197/1‑48 (MQ=255)                             ttGCTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCtgatg               <  1:1588389/48‑1 (MQ=255)                                cTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACtt           <  1:1412241/49‑1 (MQ=255)                                  gACGGCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTa         >  1:1473014/1‑49 (MQ=255)                                     ggCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTc      >  1:1559171/1‑49 (MQ=255)                                     ggCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTc      <  1:1608084/49‑1 (MQ=255)                                      gCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTCt     <  1:616935/49‑1 (MQ=255)                                      gCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTCt     <  1:1286850/49‑1 (MQ=255)                                         ttGACCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTCTGAc  <  1:33579/49‑1 (MQ=255)                                         ttGACCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTCTGAc  <  1:1715857/49‑1 (MQ=255)                                            |                                              GGCGCGGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGGCCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTCTGAC  >  NC_000913/1776490‑1776578

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑