Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,811,208	T→C	V92A (GTT→GCT)	tcyP →	cystine/sulfocysteine:cation symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,811,208	0	T	C	100.0%	93.3 / NA	25	V92A (GTT→GCT)	tcyP	cystine/sulfocysteine:cation symporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/18);  total (7/18)

ATGATCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGTTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGTTTTCT  >  NC_000913/1811165‑1811253                                            |                                              aTGATCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGccc                                          >  1:1744722/1‑49 (MQ=255) aTGATCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGccc                                          <  1:944616/49‑1 (MQ=255)  tGATCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCg                                         <  1:2087430/49‑1 (MQ=255)    aTCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTc                                       <  1:684168/49‑1 (MQ=255)     tCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCt                                      <  1:1912168/49‑1 (MQ=255)       gTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTg                                     >  1:1264505/1‑48 (MQ=255)          aTGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCAt                                  <  1:448468/48‑1 (MQ=255)          aTGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCAt                                  >  1:117988/1‑48 (MQ=255)          aTGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCAt                                  <  1:1521838/48‑1 (MQ=255)           tGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATa                                 <  1:1222616/48‑1 (MQ=255)              cGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACg                              >  1:1415689/1‑48 (MQ=255)                ttAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCAt                           <  1:799530/49‑1 (MQ=255)                ttAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCAt                           >  1:878316/1‑49 (MQ=255)                   gTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCAtctc                        <  1:1479064/49‑1 (MQ=255)                   gTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCAtctc                        <  1:1649086/49‑1 (MQ=255)                   gTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCAtctc                        <  1:358512/49‑1 (MQ=255)                        cGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGtt                    <  1:554672/48‑1 (MQ=255)                         gCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAg                  <  1:1405477/49‑1 (MQ=255)                         gCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAg                  <  1:833810/49‑1 (MQ=255)                          ccTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAgg                 <  1:1447609/49‑1 (MQ=255)                             ctATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCa               >  1:842442/1‑48 (MQ=255)                                ttCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCaaaa            <  1:1438258/48‑1 (MQ=255)                                  cTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATc          <  1:1376044/48‑1 (MQ=255)                                       cgcgGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGtttt    >  1:1411169/1‑49 (MQ=255)                                         cgGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGTTTTCt  <  1:72049/49‑1 (MQ=255)                                            |                                              ATGATCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGTTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGTTTTCT  >  NC_000913/1811165‑1811253

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑