Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,898,152	G→A	A407A (GCG→GCA)	sdaA →	L‑serine deaminase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,898,152	0	G	A	100.0%	110.8 / NA	28	A407A (GCG→GCA)	sdaA	L‑serine deaminase I
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/10);  total (18/10)

GCATTGAGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCGCGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGTCTCGCTG  >  NC_000913/1898106‑1898194                                               |                                           gCATTGAGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCcgcacg                                          >  1:1594546/1‑49 (MQ=255)  cATTGAGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCACgg                                         <  1:1594748/49‑1 (MQ=255)   aTTGAGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCACGGa                                        >  1:550186/1‑49 (MQ=255)     tGAGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCACGGATg                                      >  1:485484/1‑49 (MQ=255)     tGAGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCACGGATg                                      >  1:2094027/1‑49 (MQ=255)      gAGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCACGGATg                                      <  1:1140334/48‑1 (MQ=255)       aGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCACGGATGGc                                    <  1:1974424/49‑1 (MQ=255)         cGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCACGGATGGctc                                  >  1:1686485/1‑49 (MQ=255)           tAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCACGGATGGCTCTg                                >  1:187620/1‑49 (MQ=255)            aaTGCCATTGCCTCTGTGAAGGCGATTAACGCCGCACGGATGGCTCTgc                               >  1:1807236/1‑49 (MQ=255)                  aTTGCCTCTGTGAAGGCGATTAACGCCGCACGGATGGCTCTGCGCCGCa                         <  1:1776329/49‑1 (MQ=255)                   ttGCCTCTGTGAAGGCGATTAACGCCGCACGGATGGCTCTGCGCCGCAc                        >  1:1213443/1‑49 (MQ=255)                     gCCTCTGTGAAGGCGATTAACGCCGCACGGATGGCTCTGCGCCGCAcc                       <  1:312935/48‑1 (MQ=255)                     gCCTCTGTGAAGGCGATTAACGCCGCACGGATGGCTCTGCGCCGCAc                        >  1:451007/1‑47 (MQ=255)                      ccTCTGTGAAGGCGATTAACGCCGCACGGATGGCTCTGCGCCGCACCAg                     <  1:1691425/49‑1 (MQ=255)                           gtgAAGGCTATTAACGCCGCACGGATGGCTCTGCGCCGCACCAGTGCAc                >  1:1213504/1‑49 (MQ=25)                           gtgAAGGCGATTAACGCCGCACGGATGGCTCTGCGCCGCACCAGTGCAc                >  1:1464812/1‑49 (MQ=255)                            tgAAGGCGATTAACGCCGCACGGATGGCTCTGCGCCGCACCAGTGCAcc               >  1:150509/1‑49 (MQ=255)                               aGGCGATTAACGCCGCACGGATGGCTCTGCACCGCACCAGTGCACcgcg            >  1:1455937/1‑49 (MQ=37)                                ggCGATTAACGCCGCACGGATGGCTCTGCGCCGCACCAGTGCACcgcgc           >  1:2050526/1‑49 (MQ=255)                                  cGATTAACGCCGCACGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGt         >  1:50245/1‑49 (MQ=255)                                   gATTAACGCCGCACGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGtc        >  1:1525271/1‑49 (MQ=255)                                     ttAACGCCGCACGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGtctc      <  1:1842954/49‑1 (MQ=255)                                     ttAACGCCGCACGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGtctc      <  1:1287483/49‑1 (MQ=255)                                      tAACGCCGCACGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGTCTCg     >  1:862343/1‑49 (MQ=255)                                        aCGCCGCACGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGTCTCGCt   <  1:319122/49‑1 (MQ=255)                                         cgccgcACGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGTCTCGCTg  <  1:180818/49‑1 (MQ=255)                                         cgccgcACGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGTCTCGCTg  >  1:1632492/1‑49 (MQ=255)                                          gccgcACGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGTCTCGCTg  >  1:1727133/1‑48 (MQ=255)                                               |                                           GCATTGAGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCGCGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGTCTCGCTG  >  NC_000913/1898106‑1898194

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑