Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,941,784	A→G	V267A (GTC→GCC)	znuA ←	Zn(2(+)) ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,941,784	0	A	G	100.0%	61.8 / NA	17	V267A (GTC→GCC)	znuA	Zn(2(+)) ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (8/9);  total (8/9)

CCCAGGGGATCCAACGTTCCCATACGAACGGATGTCCCTCGTGCGACGCTCTCAACGACCGCTGGCCTGAACTGTGGCTCAGCA  >  NC_000913/1941739‑1941822                                              |                                       cccAGGGGATCCAACGTTCCCATACGAACGGATGTCCCTCGTgcggcg                                      <  1:659812/48‑1 (MQ=255)      gggATCCAACGTTCCCATACGAACGGATGTCCCTCGTGCGGCGCTCTCa                                <  1:344989/49‑1 (MQ=255)       ggATCCAACGTTCCCATACGAACGGATGTCCCTCGTGCGGCGCTCTCaa                               >  1:1091554/1‑49 (MQ=255)        gATCCAACGTTCCCATACGAACGGATGTCCCTCGTGCGGCGCTCTCAAc                              <  1:157913/49‑1 (MQ=255)         aTCCAACGTTCCCATACGAACGGATGTCCCTCGTGCGGCGCTCTCAAc                              >  1:1817678/1‑48 (MQ=255)            cAACGTTCCCATACGAACGGATGTCCCTCGTGCGGCGCTCTCAACGAc                           <  1:1542182/48‑1 (MQ=255)                gTTCCCATACGAACGGATGTCCCTCGTGCGGCGCTCTCAACGACCGCTg                      >  1:699708/1‑49 (MQ=255)                gTTCCCATACGAACGGATGTCCCTCGTGCGGCGCTCTCAACGACCGCTg                      >  1:1875663/1‑49 (MQ=255)                 ttCCCATACGAACGGATGTCCCTCGTGCGGCGCTCTCAACGACCGCTgg                     >  1:1379695/1‑49 (MQ=255)                    ccATACGAACGGATGTCCCTCGTGCGGCGCTCTCAACGACCGCTGGcc                   <  1:2097633/48‑1 (MQ=255)                    ccATACGAACGGATGTCCCTCGTGCGGCGCTCTCAACGACCGCTGGCCt                  >  1:2091635/1‑49 (MQ=255)                       tACGAACGGATGTCCCTCGTGCGGCGCTCTCAACGACCGCTGGCCTGaa               <  1:312885/49‑1 (MQ=255)                          gAACGGATGTCCCTCGTGCGGCGCTCTCAACGACCGCTGGCCTGAACtg            >  1:1027781/1‑49 (MQ=255)                              ggATGTCCCTCGTGCGGCGCTCTCAACGACCGCTGGCCTGAACTGTGGc        >  1:1931211/1‑49 (MQ=255)                                 tGTCCCTCGTGCGGCGCTCTCAACGACCGCTGGCCTGAACTGTGGCTCa     <  1:293992/49‑1 (MQ=255)                                   tCCCTCGTGCGGCGCTCTCAACGACCGCTGGCCTGAACTGTGGCTCAg    <  1:486679/48‑1 (MQ=255)                                   tCCCTCGTGCGGCGCTCTCAACGACCGCTGGCCTGAACTGTGGCTCAg    <  1:499679/48‑1 (MQ=255)                                     ccTCGTGCGGCGCTCTCAACGACCGCTGGCCTGAACTGTGGCTCAGCa  <  1:1310907/48‑1 (MQ=255)                                              |                                       CCCAGGGGATCCAACGTTCCCATACGAACGGATGTCCCTCGTGCGACGCTCTCAACGACCGCTGGCCTGAACTGTGGCTCAGCA  >  NC_000913/1941739‑1941822

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑