Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,040,801	T→C	L107L (TTA→CTA)	msrQ →	periplasmic protein‑L‑methionine sulfoxide reductase heme binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,040,801	0	T	C	100.0%	84.9 / NA	22	L107L (TTA→CTA)	msrQ	periplasmic protein‑L‑methionine sulfoxide reductase heme binding subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/11);  total (11/11)

TTTAACAAGTTACGCATTGCTGGAGCTGGGCGTGAATAATCTGGCGTTATTAGGTAAGGAGTTAATTACCCGGCCTTATTTAACGTTAGGT  >  NC_000913/2040755‑2040845                                               |                                             tttAACAAGTTACGCATTGCTGGAGCTGGGCGTGAATAATCTGGCGcta                                            <  1:915315/49‑1 (MQ=255)  ttAACAAGTTACGCATTGCTGGAGCTGGGCGTGAATAATCTGGCGctat                                           >  1:1247739/1‑49 (MQ=255)   tAACAAGTTACGCATTGCTGGAGCTGGGCGTGAATAATCTGGCGctat                                           <  1:354523/48‑1 (MQ=255)      cAAGTTACGCATTGCTGGAGCTGGGCGTGAATAATCTGGCGCTATTAgg                                       <  1:1660757/49‑1 (MQ=255)       aaGTTACGCATTGCTGGAGCTGGGCGTGAATAATCTGGCGCTATTAgg                                       >  1:664408/1‑48 (MQ=255)        aGTTACGCATTGCTGGAGCTGGGCGTGAATAATCTGGCGCTATTAGGt                                      >  1:1084394/1‑48 (MQ=255)          ttACGCATTGCTGGAGCTGGGCGTGAATAATCTGGCGCTATTAGGTAAg                                   >  1:2011800/1‑49 (MQ=255)              gCATTGCTGGAGCTGGGCGTGAATAATCTGGCGCTATTAGGTAAGGAGt                               <  1:1866094/49‑1 (MQ=255)                 ttGCTGGAGCTGGGCGTGAATAATCTGGCGCTATTAGGTAAGGAGTTaa                            <  1:484486/49‑1 (MQ=255)                   gCTGGAGCTGGGCGTGAATAATCTGGCGCTATTAGGTAAGGAGTTAAt                           <  1:1534485/48‑1 (MQ=255)                    cTGGAGCTGGGCGTGAATAATCTGGCGCTATTAGGTAAGGAGTTAAtt                          <  1:1327984/48‑1 (MQ=255)                        aGCTGGGCGTGAATAATCTGGCGCTATTAGGTAAGGAGTTAATTACCCg                     >  1:1312931/1‑49 (MQ=255)                        aGCTGGGCGTGAATAATCTGGCGCTATTAGGTAAGGAGTTAATTACCCg                     <  1:1384099/49‑1 (MQ=255)                         gCTGGGCGTGAATAATCTGGCGCTATTAGGTAAGGAGTTAATTACCCgg                    <  1:847679/49‑1 (MQ=255)                           tGGGCGTGAATAATCTGGCGCTATTAGGTAAGGAGTTAATTACCCGGcc                  >  1:2073291/1‑49 (MQ=255)                              gCGTGAATAATCTGGCGCTATTAGGTAAGGAGTTAATTACCCGGCCTTa               <  1:631428/49‑1 (MQ=255)                              gCGTGAATAATCTGGCGCTATTAGGTAAGGAGTTAATTACCCGGCCTTa               >  1:1127394/1‑49 (MQ=255)                              gCGTGAATAATCTGGCGCTATTAGGTAAGGAGTTAATTACCCGGCCTTa               <  1:29118/49‑1 (MQ=255)                                gTGAATAATCTGGCGCTATTAGGTAAGGAGTTAATTACCCGGCCTTAtt             >  1:477547/1‑49 (MQ=255)                                gTGAATAATCTGGCGCTATTAGGTAAGGAGTTAATTACCCGGCCTTAtt             >  1:1519716/1‑49 (MQ=255)                                 tGAATAATCTGGCGCTATTAGGTAAGGAGTTAATTACCCGGCCTTAttt            <  1:129239/49‑1 (MQ=255)                                 tGAATAATCTGGCGCTATTAGGTAAGGAGTTAATTACCCGGCCTTAtt             >  1:1305829/1‑48 (MQ=255)                                    ataatCTGGCGCTATTAGGTAAGGAGTTAATTACCCGGCCTTATTTAAc         >  1:267145/1‑49 (MQ=255)                                       atCTGGCGCTATTAGGTAAGGAGTTAATTACCCGGCCTTATTTAACGt       <  1:1881749/48‑1 (MQ=255)                                         cTGGCGCTATTAGGTAAGGAGTTAATTACCCGGCCTTATTTAACGTTAg    >  1:460005/1‑49 (MQ=255)                                            gcgcTATTAGGTAAGGAGTTAATTACCCGGCCTTATTTAACGTTAGGt  <  1:456400/48‑1 (MQ=255)                                            gcgcTATTAGGTAAGGAGTTAATTACCCGGCCTTATTTAACGTTAGGt  <  1:1163007/48‑1 (MQ=255)                                               |                                             TTTAACAAGTTACGCATTGCTGGAGCTGGGCGTGAATAATCTGGCGTTATTAGGTAAGGAGTTAATTACCCGGCCTTATTTAACGTTAGGT  >  NC_000913/2040755‑2040845

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑