| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 214,885 | C→T | T18I (ACC→ATC) | arfB → | peptidyl‑tRNA hydrolase, ribosome rescue factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 214,885 | 0 | C | T | 95.8% | 89.1 / ‑6.3 | 24 | T18I (ACC→ATC) | arfB | peptidyl‑tRNA hydrolase, ribosome rescue factor |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (8/15); minor base A (1/0); total (9/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.75e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAAT > NC_000913/214840‑214925 | tgATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGc < 1:968869/49‑1 (MQ=255)tgATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGc < 1:852724/49‑1 (MQ=255) gATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGc > 1:1169214/1‑48 (MQ=255) aTTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGcc < 1:1139822/48‑1 (MQ=255) aTTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGcc < 1:1510155/48‑1 (MQ=255) aTTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCa > 1:1204685/1‑49 (MQ=255) cccGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTc < 1:364853/48‑1 (MQ=255) ccGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGt > 1:1683406/1‑49 (MQ=255) cGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTg < 1:394697/49‑1 (MQ=255) cATGTTGCTATTCCCGATGGTGAGCTTGAGATAAAAGCCATTCGTggg > 1:1348393/1‑46 (MQ=255) tGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAg > 1:2061840/1‑49 (MQ=255) tGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAg < 1:1262276/49‑1 (MQ=255) gTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAgg > 1:470782/1‑49 (MQ=255) ttGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAggg < 1:2696/49‑1 (MQ=255) gCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGgc < 1:150172/48‑1 (MQ=255) cTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGgcgc < 1:1774408/49‑1 (MQ=255) aTTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCgcgg > 1:1269738/1‑49 (MQ=255) cccGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCgcgggc < 1:717050/48‑1 (MQ=255) gATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGcgggcggg > 1:1374906/1‑48 (MQ=255) tGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGgcag < 1:165858/49‑1 (MQ=255) gTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGgcagca > 1:1999577/1‑49 (MQ=255) tGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCAt < 1:947364/49‑1 (MQ=255) cTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTta < 1:2065653/49‑1 (MQ=255) gagaTCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTtaat < 1:948759/48‑1 (MQ=255) | TGATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAAT > NC_000913/214840‑214925 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |