Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,662,004 T→C E43G (GAA→GGA)  iscR ← DNA‑binding transcriptional dual regulator IscR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,662,0040TC100.0% 64.2 / NA 17E43G (GAA→GGA) iscRDNA‑binding transcriptional dual regulator IscR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/9);  total (8/9)

CGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCC  >  NC_000913/2661960‑2662048
                                            |                                            
cGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCa                                           <  1:41215/48‑1 (MQ=255)
     gAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGAt                                        >  1:1106724/1‑46 (MQ=255)
     gAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGAt                                        >  1:69725/1‑46 (MQ=255)
     gAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATaa                                      <  1:1137209/48‑1 (MQ=255)
     gAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAg                                     <  1:429837/49‑1 (MQ=255)
      aaaCCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAg                                     >  1:1811771/1‑48 (MQ=255)
      aaaCCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGa                                    <  1:587627/49‑1 (MQ=255)
              ccATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGaaa                            <  1:485458/49‑1 (MQ=255)
                aTTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAAtt                          <  1:935561/49‑1 (MQ=255)
                 tttttACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTc                         <  1:1684452/49‑1 (MQ=255)
                     tACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCt                      >  1:236631/1‑48 (MQ=255)
                        gCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACg                  <  1:1008642/49‑1 (MQ=255)
                           gACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTc               >  1:40457/1‑49 (MQ=255)
                            aCGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCg              >  1:1237069/1‑49 (MQ=255)
                               ggAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGa            >  1:781988/1‑48 (MQ=255)
                                   aaCAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAatat       >  1:674223/1‑49 (MQ=255)
                                    aCAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATc      <  1:884141/49‑1 (MQ=255)
                                       gttgtcCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGc   >  1:670672/1‑49 (MQ=255)
                                         tgtcCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGcc  >  1:531884/1‑48 (MQ=255)
                                            |                                            
CGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCC  >  NC_000913/2661960‑2662048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑