Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	257,794	T→C	intergenic (+23/‑35)	frsA → / → crl	fermentation‑respiration switch protein/RNA polymerase holoenzyme assembly factor Crl

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	257,794	0	T	C	100.0%	156.9 / NA	39	intergenic (+23/‑35)	frsA/crl	fermentation‑respiration switch protein/RNA polymerase holoenzyme assembly factor Crl
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/14);  total (25/14)

TGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTAC  >  NC_000913/257748‑257838                                               |                                             tGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTg                                            >  1:1544044/1‑49 (MQ=255)   gATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGt                                          <  1:265022/49‑1 (MQ=255)    aTCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGt                                          >  1:56203/1‑48 (MQ=255)    aTCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTa                                         <  1:1190222/49‑1 (MQ=255)       gAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaaa                                       <  1:789274/48‑1 (MQ=255)       gAAAAACGCTTGTGTTAAAAATTTGCTAAAGTTTGCCAATCTGGTaaaa                                      <  1:1491152/49‑1 (MQ=38)        aaaaaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaaaa                                      >  1:590989/1‑48 (MQ=255)        aaaaaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaaaa                                      >  1:132952/1‑48 (MQ=255)        aaaaaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaaaa                                      >  1:506619/1‑48 (MQ=255)          aaaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAg                                   >  1:672511/1‑49 (MQ=255)           aaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGt                                  >  1:1671949/1‑49 (MQ=255)           aaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGt                                  <  1:494555/49‑1 (MQ=255)           aaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGt                                  >  1:18419/1‑49 (MQ=255)            aCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGtt                                 >  1:219738/1‑49 (MQ=255)             cGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTg                                <  1:1588849/49‑1 (MQ=255)             cGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTg                                >  1:545904/1‑49 (MQ=255)              gCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTg                                >  1:1270889/1‑48 (MQ=255)               cTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCa                              >  1:1417198/1‑49 (MQ=255)               cTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCa                              <  1:1737026/49‑1 (MQ=255)                  gtgtTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATca                           >  1:861100/1‑49 (MQ=255)                  gtgtTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATca                           >  1:128105/1‑49 (MQ=255)                  gtgtTAAAAATTTGCTAAATTTTGCCAATCTGGCAAAACAGTTGCATca                           >  1:170546/1‑49 (MQ=39)                   tgtTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATca                           >  1:1291961/1‑48 (MQ=255)                     ttAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCacaa                        <  1:1936202/49‑1 (MQ=255)                       aaaaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCacaac                       <  1:424114/48‑1 (MQ=255)                         aaaTTTGCTAAATTTTGTCAATCTGGTAAAACAGTTGCATCACAACAgg                    <  1:449741/49‑1 (MQ=38)                            tttGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAg                     >  1:248983/1‑45 (MQ=255)                               gCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAg              >  1:1856465/1‑49 (MQ=255)                                cTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAg              >  1:1469126/1‑48 (MQ=255)                                cTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAg              >  1:641671/1‑48 (MQ=255)                                  aaaTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCaa           >  1:127909/1‑49 (MQ=255)                                  aaaTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCaa           >  1:271282/1‑49 (MQ=255)                                   aaTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCaa           <  1:1094704/48‑1 (MQ=255)                                        tGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGt     >  1:350394/1‑49 (MQ=255)                                          ccAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGtt    <  1:1631962/48‑1 (MQ=255)                                          ccAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTa   >  1:1774666/1‑49 (MQ=255)                                          ccAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTa   <  1:2057920/49‑1 (MQ=255)                                           cAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTAc  <  1:751072/49‑1 (MQ=255)                                           cAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTAc  >  1:1891972/1‑49 (MQ=255)                                               |                                             TGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTAC  >  NC_000913/257748‑257838

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑