Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,813,364	T→C	V250A (GTC→GCC)	emrB →	multidrug efflux pump membrane subunit EmrB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,813,364	0	T	C	100.0%	92.0 / NA	24	V250A (GTC→GCC)	emrB	multidrug efflux pump membrane subunit EmrB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/14);  total (10/14)

TCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAACCCGATAGTCGATCTGTCGT  >  NC_000913/2813319‑2813405                                              |                                          tcatcCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGCCt                                         <  1:1743050/48‑1 (MQ=255)   atcCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGCCTggg                                      <  1:1081800/49‑1 (MQ=255)     ccTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGCCTGGGAg                                    >  1:1366078/1‑49 (MQ=255)      cTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGCCTGGGAGc                                   >  1:1159692/1‑49 (MQ=255)       ttACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGCCTGGGAGCt                                  >  1:1814863/1‑49 (MQ=255)         aCCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGCCTGGGAGCTGa                                >  1:945212/1‑49 (MQ=255)          ccGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGCCTGGGAGCTGa                                <  1:726793/48‑1 (MQ=255)           cGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGCCTGGGAGCTGAcc                              >  1:999017/1‑49 (MQ=255)            gtggtgGCGGTGGTGGCTATCTGCTTCCTGATTGCCTGGGAGCTGAcc                              >  1:565316/1‑48 (MQ=255)            gtggtgGCGGTGGTGGCTATCTGCTTCCTGATTGCCTGGGAGCTGACcg                             <  1:1457853/49‑1 (MQ=255)               gtggCGGTGGTGGCTATCTGCTTCCTGATTGCCTGGGAGCTGACcgac                           <  1:12090/48‑1 (MQ=255)                tggCGGTGGTGGCTATCTGCTTCCTGATTGCCTGGGAGCTGACcgacga                         <  1:156944/49‑1 (MQ=255)                  gCGGTGGTGGCTATCTGCTTCCTGATTGCCTGGGAGCTGACCGACGAt                        <  1:384658/48‑1 (MQ=255)                   cGGTGGTGGCTATCTGCTTCCTGATTGCCTGGGAGCTGACCGACGATaa                      >  1:945105/1‑49 (MQ=255)                    ggtggtGGCTATCTGCTTCCTGATTGCCTGGGAGCTGACCGACGATAAc                     >  1:2080622/1‑49 (MQ=255)                     gtggtgGCTATCTGCTTCCTGATTGCCTGGGAGCTGACCGACGATAAcc                    >  1:1452015/1‑49 (MQ=255)                        gtggCTATCTGCTTCCTGATTGCCTGGGAGCTGACCGACGATAACCCGa                 <  1:843538/49‑1 (MQ=255)                        gtggCTATCTGCTTCCTGATTGCCTGGGAGCTGACCGACGATAACCCGa                 <  1:1684253/49‑1 (MQ=255)                           gCTATCTGCTTCCTGATTGCCTGGGAGCTGACCGACGATAACCCGATa               <  1:549351/48‑1 (MQ=255)                            cTATCTGCTTCCTGATTGCCTGGGAGCTGACCGACGATAACCCGATAGt             <  1:655102/49‑1 (MQ=255)                               tCTGCTTCCTGATTGCCTGGGAGCTGACCGACGATAACCCGATAGTc            <  1:1426019/47‑1 (MQ=255)                               tCTGCTTCCTGATTGCCTGGGAGCTGACCGACGATAACCCGATAGTc            <  1:135593/47‑1 (MQ=255)                               tCTGCTTCCTGATTGCCTGGGAGCTGACCGACGATAACCCGATAGTc            <  1:963001/47‑1 (MQ=255)                                       cTGATTGCCTGGGAGCTGACCGACGATAACCCGATAGTCGATCTGTCgt  >  1:1752936/1‑49 (MQ=255)                                              |                                          TCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAACCCGATAGTCGATCTGTCGT  >  NC_000913/2813319‑2813405

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑