Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,843,412	C→T	R12H (CGT→CAT)	hycH ←	formate hydrogenlyase assembly protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,843,412	0	C	T	100.0%	67.2 / NA	18	R12H (CGT→CAT)	hycH	formate hydrogenlyase assembly protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/11);  total (7/11)

CTGCGCCTCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTACGGCTCAGTTGACTGAACACCACCTTTTCACTCATCGGATACGCG  >  NC_000913/2843369‑2843456                                            |                                             ctgcGCCTCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTATGGCt                                          <  1:1811169/48‑1 (MQ=255)    cgcCTCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTATGGCTCAg                                       <  1:886638/48‑1 (MQ=255)    cgcCTCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTATGGCTCAGt                                      <  1:775999/49‑1 (MQ=255)    cgcCTCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTATGGCTCAGt                                      <  1:522673/49‑1 (MQ=255)          ggcgggcgTGGCATCGTTCTCATCAATAAATTTATGGCTCAGTTGACt                                 <  1:1260484/48‑1 (MQ=255)            cgggcgTGGCATCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGaa                              <  1:1612950/49‑1 (MQ=255)             gggcgTGGCATCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGaa                              >  1:1451481/1‑48 (MQ=255)             gggcgTGGCATCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGAac                             <  1:193007/49‑1 (MQ=255)                  tGGCATCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGAAcaccac                        >  1:685586/1‑49 (MQ=255)                   ggCATCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGAACaccacc                       >  1:1947324/1‑49 (MQ=255)                         gTTCTCATCAATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCa                 >  1:1669813/1‑49 (MQ=255)                            ctcATCAATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCACt               <  1:1805421/48‑1 (MQ=255)                               atcaATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCACTCa             <  1:92967/47‑1 (MQ=255)                               atcaATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCACTCATc           <  1:1241682/49‑1 (MQ=255)                                 caATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCACTCATCg          >  1:1687419/1‑48 (MQ=255)                                     aaaTTTATGGCTCAGTTGACTGAACACCACCTTTTCACTCATCGGATAc     >  1:665949/1‑49 (MQ=255)                                      aaTTTATGGCTCAGTTGACTGAACACCACCTTTTCACTCATCGGATAc     <  1:989602/48‑1 (MQ=255)                                        tttATGGCTCAGTTGACTGAACACCACCTTTTCACTCATCGGATAcgcg  >  1:1426080/1‑49 (MQ=255)                                            |                                             CTGCGCCTCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTACGGCTCAGTTGACTGAACACCACCTTTTCACTCATCGGATACGCG  >  NC_000913/2843369‑2843456

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑