Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,090,282	C→T	intergenic (+30/‑65)	yggI → / → endA	conserved protein YggI/DNA‑specific endonuclease I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,090,282	0	C	T	100.0%	135.3 / NA	34	intergenic (+30/‑65)	yggI/endA	conserved protein YggI/DNA‑specific endonuclease I
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/18);  total (16/18)

AGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCT  >  NC_000913/3090236‑3090325                                               |                                            aGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATTTg                                           <  1:1982873/49‑1 (MQ=255)      gTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATTTGGCTg                                       <  1:469162/48‑1 (MQ=255)       ttGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATTTGGCTGAt                                     >  1:610475/1‑49 (MQ=255)          cGAAATAACCATCTGAACTATCAGGAACTTTCCTGATTTGGCTGATTg                                   >  1:1903197/1‑48 (MQ=255)           gAAATAACCATCTGAACTATCAGGAACTTTCCTGATTTGGCTGATTGCa                                 >  1:1067026/1‑49 (MQ=255)           gAAATAACCATCTGAACTATCAGGAACTTTCCTGATTTGGCTGATTGCa                                 <  1:28725/49‑1 (MQ=255)            aaaTAACCATCTGAACTATCAGGAACTTTCCTGATTTGGCTGATTGCa                                 >  1:1945968/1‑48 (MQ=255)               tAACCATCTGAACTATCAGGAACTTTCCTGATTTGGCTGATTGCATAc                              >  1:216781/1‑48 (MQ=255)               tAACCATCTGAACTATCAGGAACTTTCCTGATTTGGCTGATTGCATAc                              >  1:2044524/1‑48 (MQ=255)               tAACCATCTGAACTATCAGGAACTTTCCTGATTTGGCTGATTGCATAc                              >  1:1792461/1‑48 (MQ=255)                 aCCATCTGAACTATCAGGAACTTTCCTGATTTGGCTGATTGCATACCa                            <  1:1150431/48‑1 (MQ=255)                 aCCATCTGAACTATCAGGAACTTTCCTGATTTGGCTGATTGCATACCa                            >  1:784802/1‑48 (MQ=255)                  ccATCTGAACTATCAGGAACTTTCCTGATTTGGCTGATTGCATACCaaa                          <  1:1110365/49‑1 (MQ=255)                  ccATCTGAACTATCAGGAACTTTCCTGATTTGGCTGATTGCATACCaa                           >  1:581147/1‑48 (MQ=255)                   cATCTGAACTATCAGGAACTTTCCTGATTTGGCTGATTGCATACCaaaa                         <  1:983304/49‑1 (MQ=255)                       tGAACTATCAGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGc                     >  1:1661181/1‑49 (MQ=255)                         aaCTATCAGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACAg                      <  1:1299117/46‑1 (MQ=255)                         aaCTATCAGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACAg                      <  1:1586469/46‑1 (MQ=255)                         aaCTATCAGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACAg                      <  1:211181/46‑1 (MQ=255)                         aaCTATCAGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCtt                   >  1:659158/1‑49 (MQ=255)                          aCTATCAGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCttt                  <  1:183332/49‑1 (MQ=255)                           cTATCAGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCTTTc                 >  1:1635370/1‑49 (MQ=255)                           cTATCAGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCTTTc                 <  1:1641075/49‑1 (MQ=255)                           cTATCAGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCTTTc                 <  1:709536/49‑1 (MQ=255)                            tATCAGGAACTTTCCTGATTTGGCTGTTTGCATACCAAAACAGCTTTCg                >  1:1225175/1‑49 (MQ=255)                            tATCAGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCTTTCg                >  1:1890686/1‑49 (MQ=255)                              tCAGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCTTTCGCt              <  1:898042/49‑1 (MQ=255)                                aGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCTTTCGCTAc            <  1:524757/49‑1 (MQ=255)                                  gAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCTTTCGCTACGt          >  1:1377405/1‑49 (MQ=255)                                   aaCTTTCCTGATTTGGCTGATTGCATACCAAAACAGCTTTCGCTACGtt         <  1:146206/49‑1 (MQ=255)                                   aaCTTTCCTGATTTGGCTGATTGCATACCAAAACAGCTTTCGCTACGtt         <  1:1589853/49‑1 (MQ=255)                                     cTTTCCTGATTTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGc       <  1:1270104/49‑1 (MQ=255)                                        tCCTGATTTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTgg    >  1:272707/1‑49 (MQ=255)                                          cTGATTTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCt  <  1:624449/49‑1 (MQ=255)                                               |                                            AGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCT  >  NC_000913/3090236‑3090325

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑