Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,513,003	A→G	D212D (GAT→GAC)	trpS ←	tryptophan‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,513,003	0	A	G	100.0%	69.8 / NA	19	D212D (GAT→GAC)	trpS	tryptophan‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/9);  total (10/9)

CGGAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGATCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGACT  >  NC_000913/3512957‑3513046                                               |                                            cgGAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTc                                           >  1:1690146/1‑49 (MQ=255)  ggAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCt                                          <  1:1118709/49‑1 (MQ=255)  ggAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCt                                          >  1:410859/1‑49 (MQ=255)     gTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTcc                                       <  1:775893/49‑1 (MQ=255)      tCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCca                                      <  1:1840907/49‑1 (MQ=255)        aGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCcag                                     >  1:762626/1‑48 (MQ=255)         gTGACCGCACGTGTGATTTTCTTCACGACCGATTTCGGGTCTTCcagca                                   <  1:1352077/49‑1 (MQ=38)                   gTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAAc                         >  1:1633380/1‑49 (MQ=255)                   gTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAAc                         <  1:875968/49‑1 (MQ=255)                    tttGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACg                        >  1:1420816/1‑49 (MQ=255)                        aTTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTAt                    <  1:771191/49‑1 (MQ=255)                         ttttCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTAtt                   <  1:345923/49‑1 (MQ=255)                          tttCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTg                  >  1:297463/1‑49 (MQ=255)                                cACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTa            >  1:1518062/1‑49 (MQ=255)                                 aCTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTAt           <  1:723082/49‑1 (MQ=255)                                 aCTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTAt           >  1:848132/1‑49 (MQ=255)                                 aCTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTAt           <  1:870526/49‑1 (MQ=255)                                      cGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGt        >  1:872523/1‑47 (MQ=255)                                          ttCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGACt  >  1:544914/1‑49 (MQ=255)                                               |                                            CGGAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGATCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGACT  >  NC_000913/3512957‑3513046

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑