Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,816,579	G→A	intergenic (‑30/‑97)	rph ← / → yicC	truncated RNase PH/UPF0701 family protein YicC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,816,579	0	G	A	100.0%	97.8 / NA	25	intergenic (‑30/‑97)	rph/yicC	truncated RNase PH/UPF0701 family protein YicC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/13);  total (12/13)

CGGCCTGCTGGACGCATATTGAAATCTCCGGCTTGAAACAAATGTGGCTGCGCATTATACGGACTTCCGGCGGTTATTCCTATCCTGA  >  NC_000913/3816533‑3816620                                               |                                          cGGCCTGCTGGACGCATATTGAAATCTCCGGCTTGAAACAAATGTGACt                                         <  1:720382/49‑1 (MQ=255)  ggCCTGCTGGACGCATATTGAAATCTCCGGCTTGAAACAAATGTGACTg                                        <  1:1281121/49‑1 (MQ=255)   gCCTGCTGGACGCATATTGAAATCTCCGGCTTGAAACAAATGTGACTgc                                       >  1:1425165/1‑49 (MQ=255)    ccTGCTGGACGCATATTGAAATCTCCGGCTTGAAACAAATGTGACTgcg                                      <  1:1981485/49‑1 (MQ=255)        ctgGACGCATATTGAAATCTCCGGCTTGAAACAAATGTGACTGCGCa                                    <  1:144268/47‑1 (MQ=255)        ctgGACGCATATTGAAATCTCCGGCTTGAAACAAATGTGACTGCGCa                                    <  1:1947953/47‑1 (MQ=255)        ctgGACGCATATTGAAATCTCCGGCTTGAAACAAATGTGACTGCGCAt                                   >  1:1765343/1‑48 (MQ=255)         tgGACGCATATTGAAATCTCCGGCTTGAAACAAATGTGACTGCGCATta                                 >  1:1608402/1‑49 (MQ=255)         tgGACGCATATTGAAATCTCCGGCTTGAAACAAATGTGACTGCGCATta                                 >  1:1926321/1‑49 (MQ=255)          ggACGCATATTGAAATCTCCGGCTTGAAACAAATGTGACTGCGCATta                                 >  1:151542/1‑48 (MQ=255)           gACGCATATTGAAATCTCCGGCTTGAAACAAATGTGACTGCGCATtat                                <  1:1092559/48‑1 (MQ=255)            aCGCATATTGAAATCTCCGGCTTGAAACAAATGTGACTGCGCATTATAc                              <  1:575478/49‑1 (MQ=255)            aCGCATATTGAAATCTCCGGCTTGAAACAAATGTGACTGCGCATTATAc                              <  1:31956/49‑1 (MQ=255)                 tatTGAAATCTCCGGCTTGAAACAAATGTGACTGCGCATTATACGGACt                         >  1:1443015/1‑49 (MQ=255)                  atTGAAATCTCCGGCTTGAAACAAATGTGACTGCGCATTATACGGACtt                        >  1:1942372/1‑49 (MQ=255)                   ttGAAATCTCCGGCTTGAAACAAATGTGACTGCGCATTATACGGACtt                        <  1:77299/48‑1 (MQ=255)                       aaTCTCCGGCTTGAAACAAATGTGACTGCGCATTATACGGACTTCcggc                   >  1:1764810/1‑49 (MQ=255)                           tcCGGCTTGAAACAAATGTGACTGCGCATTATACGGACTTCCGGCGGt                >  1:1586297/1‑48 (MQ=255)                             cGGCTTGAAACAAATGTGACTGCGCATTATACGGACTTCcggcgg                 <  1:1709019/45‑1 (MQ=255)                             cGGCTTGAAACAAATGTGACTGCGCATTATACGGACTTCCGGCGGTTAt             >  1:120185/1‑49 (MQ=255)                              ggCTTGAAACAAATGTGACTGCGCATTATACGGACTTCCGGCGGTTAtt            <  1:450662/49‑1 (MQ=255)                                       cAAATGTGACTGCGCATTATACGGACTTCCGGCGGTTATTCCTATCCt    >  1:707690/1‑48 (MQ=255)                                        aaaTGTGACTGCGCATTATACGGACTTCCGGCGGTTATTCCTATCCTGa  <  1:443344/49‑1 (MQ=255)                                        aaaTGTGACTGCGCATTATACGGACTTCCGGCGGTTATTCCTATCCTGa  <  1:1599863/49‑1 (MQ=255)                                         aaTGTGACTGCGCATTATACGGACTTCCGGCGGTTATTCCTATCCTGa  >  1:2024322/1‑48 (MQ=255)                                               |                                          CGGCCTGCTGGACGCATATTGAAATCTCCGGCTTGAAACAAATGTGGCTGCGCATTATACGGACTTCCGGCGGTTATTCCTATCCTGA  >  NC_000913/3816533‑3816620

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑