Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,850,891	G→A	Q68* (CAA→TAA)	ilvN ←	acetohydroxy acid synthase I subunit IlvN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,850,891	0	G	A	100.0%	97.9 / NA	25	Q68* (CAA→TAA)	ilvN	acetohydroxy acid synthase I subunit IlvN
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/14);  total (11/14)

CTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTGGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGCC  >  NC_000913/3850847‑3850935                                             |                                             cTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCt                                           >  1:1462914/1‑48 (MQ=255)  tGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCTTa                                         >  1:150354/1‑49 (MQ=255)       aCGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCTTatcat                                     <  1:1832043/48‑1 (MQ=255)        cGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCTTATCATCt                                   >  1:1306426/1‑49 (MQ=255)         gCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCTTATCATCTg                                  <  1:998699/49‑1 (MQ=255)             cACTTTCACGACATCTTCCAGCTTATCGATTTAGCTTATCATCTGCTcc                              <  1:491479/49‑1 (MQ=255)             cACTTTCACGACATCTTCCAGCTTATCGATTTAGCTTATCATCTGCTc                               >  1:1532512/1‑48 (MQ=255)             cACTTTCACGACATCTTCCAGCTTATCGATTTAGCTTATCATCTGCTc                               >  1:1259455/1‑48 (MQ=255)               cTTTCACGACATCTTCCAGCTTATCGATTTAGCTTATCATCTGCTcctc                            <  1:1274176/49‑3 (MQ=39)                  tCACGACATCTTCCAGCTTATCGATTTAGCTTATCATCTGCTCCAGACg                         >  1:1812116/1‑49 (MQ=255)                     cGACATCTTCCAGCTTATCGATTTAGCTTATCATCTGCTCCAGACGCTg                      >  1:170295/1‑49 (MQ=255)                      gACATCTTCCAGCTTATCGATTTAGCTTATCATCTGCTCCAGACGCTgg                     <  1:1814972/49‑1 (MQ=255)                      gACATCTTCCAGCTTATCGATTTAGCTTATCATCTGCTCCAGACGCTgg                     <  1:343664/49‑1 (MQ=255)                        cATCTTCCAGCTTATCGATTTAGCTTATCATCTGCTCCAGACGCTGgtc                   >  1:1806088/1‑49 (MQ=255)                        cATCTTCCAGCTTATCGATTTAGCTTATCATCTGCTCCAGACGCTGgtc                   <  1:1700784/49‑1 (MQ=255)                          tCTTCCAGCTTATCGATTTAGCTTATCATCTGCTCCAGACGCTGgtcgt                 >  1:1994503/1‑49 (MQ=255)                              ccAGCTTATCGATTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCAt              <  1:83659/48‑1 (MQ=255)                                aGCTTATCGATTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGa           <  1:1743858/49‑1 (MQ=255)                                 gCTTATCGATTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGAc          <  1:504056/49‑1 (MQ=255)                                      tCGATTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAg       >  1:246733/1‑47 (MQ=255)                                      tCGATTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGt      <  1:575070/48‑1 (MQ=255)                                      tCGATTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTa     <  1:466153/49‑1 (MQ=255)                                        gATTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGc   <  1:1783373/49‑1 (MQ=255)                                         aTTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGcc  <  1:787977/49‑1 (MQ=255)                                         aTTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGc   >  1:1263118/1‑48 (MQ=255)                                             |                                             CTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTGGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGCC  >  NC_000913/3850847‑3850935

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑