Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,871,692	C→T	R13R (CGG→CGA)	dgoT ←	putative D‑galactonate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,871,692	0	C	T	100.0%	112.0 / NA	28	R13R (CGG→CGA)	dgoT	putative D‑galactonate transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/14);  total (14/14)

AATGACTACCGTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGC  >  NC_000913/3871650‑3871737                                           |                                              aaTGACTACCGTAATAAAGATCATCACCAGCGTCAGATAACGTCGACGc                                         >  1:316476/1‑49 (MQ=255) aaTGACTACCGTAATAAAGATCATCACCAGCGTCAGATAACGTCGACGc                                         >  1:1584123/1‑49 (MQ=255)     aCTACCGTAATAAAGATCATCACCAGCGTCAGATAACGTCGACGcccc                                      <  1:336871/48‑1 (MQ=255)     aCTACCGTAATAAAGATCATCACCAGCGTCAGATAACGTCGACGCCCCg                                     <  1:1701301/49‑1 (MQ=255)       tACCGTAATAAAGATCATCACCAGCGTCAGATAACGTCGACGCCCCGGc                                   >  1:1117227/1‑49 (MQ=255)         ccGTAATAAAGATCATCACCAGCGTCAGATAACGTCGACGCCCCGGCtt                                 >  1:2027791/1‑49 (MQ=255)         ccGTAATAAAGATCATCACCAGCGTCAGATAACGTCGACGCCCCGGCt                                  <  1:289287/48‑1 (MQ=255)           gTAATAAAGATCATCACCAGCGTCAGATAACGTCGACGCCCCGGCTTtg                               >  1:624597/1‑49 (MQ=255)              ataaAGATCATCACCAGCGTCAGATAACGTCGACGCCCCGGCTTtgctg                            <  1:1094229/49‑1 (MQ=255)                  aGATCATCACCAGCGTCAGATAACGTCGACGCCCCGGCTTTGCTGCAt                         >  1:460873/1‑48 (MQ=255)                    atcatcACCAGCGTCAGATAACGTCGACGCCCCGGCTTTGCTGCATTaa                      >  1:1685325/1‑49 (MQ=255)                     tcatcaCCAGCGTCAGATAACGTCGACGCCCCGGCTTTGCTGCATTAAc                     <  1:702319/49‑1 (MQ=255)                      catcaCCAGCGTCAGATAACGTCGACGCCCCGGCTTTGCTGCATTAACg                    >  1:1492625/1‑49 (MQ=255)                       atcaCCAGCGTCAGATAACGTCGACGCCCCGGCTTTGCTGCATTAACgg                   >  1:1169478/1‑49 (MQ=255)                         caCCAGCGTCAGATAACGTCGACGCCCCGGCTTTGCTGCATTAACggg                  >  1:224384/1‑48 (MQ=255)                          aCCAGCGTCAGATAACGTCGACGCCCCGGCTTTGCTGCATTAACGGGaa                >  1:630704/1‑49 (MQ=255)                          aCCAGCGTCAGATAACGTCGACGCCCCGGCTTTGCTGCATTAACGGGaa                >  1:778877/1‑49 (MQ=255)                                gTCAGATAACGTCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCa          >  1:24860/1‑49 (MQ=255)                                 tCAGATAACGTCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCAt         <  1:1946975/49‑1 (MQ=255)                                   aGATAACGTCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATa        <  1:106103/48‑1 (MQ=255)                                   aGATAACGTCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAg       >  1:1339754/1‑49 (MQ=255)                                    gATAACGTCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGc      <  1:49013/49‑1 (MQ=255)                                     aTAACGTCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCg     <  1:1899785/49‑1 (MQ=255)                                     aTAACGTCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCg     <  1:1780892/49‑1 (MQ=255)                                        aCGTCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAg   <  1:1714445/48‑1 (MQ=255)                                        aCGTCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAg   <  1:1222611/48‑1 (MQ=255)                                        aCGTCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGc  <  1:1901523/49‑1 (MQ=255)                                        aCGTCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGc  <  1:1729431/49‑1 (MQ=255)                                           |                                              AATGACTACCGTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGC  >  NC_000913/3871650‑3871737

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑