Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,188,282	G→A	R978H (CGT→CAT)	rpoC →	RNA polymerase subunit beta'

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,188,282	0	G	A	85.7%	11.8 / NA	7	R978H (CGT→CAT)	rpoC	RNA polymerase subunit beta'
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (3/3);  minor base C (0/1);  total (3/4)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGAACAGGTTGCTGGCGGCGAAACCGTTGCAAAC  >  NC_000913/4188188‑4188406                                                                                               |                                                                                                                             gAATCCAGCAACCCAAGGGAAAACAAAGGTGGCATCAAGCTCAGGAACGTGAAATCGGTTGGGAACTCCAGCGGTAAACTGGTTATCACTTCCCATAATACTGAACTGAAACTGATCGACGAATTCGGt                                                                                            <  1:97509/129‑1 (MQ=255)                tggAAAAACAAAGGGAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTAAACGCCAGCGGTAAACTGGTTATCACTTCCCATAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGc                                                                             <  1:212352/127‑1 (MQ=255)                   aaaaaCAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCATAATACTGAACTGAAACTGATCGACGAATTCGGTCTTCCTAAAGAAAGCTAc                                                                          >  1:263081/1‑129 (MQ=255)                           aGGTAGCATCCAGCTCAGGAACGGGAAGGCGGTTGTGAACCCCAGCGGGAAACTGGTTATCACTTCCCATAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTAcc                                                                  <  1:602985/129‑1 (MQ=255)                                              aaggTGAAGGGGGTGGTGAAC‑CCAGGGGGAAAACGGTTTACCCTTCCCCAAAAACTGAACGTAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCGACAAAGTACCTTACGGTGCGGTACTGGCGa                                              <  1:159367/126‑1 (MQ=255)                                                                                 ggTTATCACTTCCCATAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTAAAAAGTACCTTACGTTGCGGACCTGGCAAAAGGCGTTGGGGAA‑AGGTGTGTGGCGGGGAAAc            >  1:213970/1‑128 (MQ=255)                                                                                          ttCCCATAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGAACTGGCGAAAGGCACTGGCGACCGGGTGCCTGAC‑GCGAAACCGTTGCAAAc  >  1:418485/1‑129 (MQ=255)                                                                                               |                                                                                                                             GAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGAACAGGTTGCTGGCGGCGAAACCGTTGCAAAC  >  NC_000913/4188188‑4188406

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑