Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	765,191	Δ1 bp	coding (685/723 nt)	mngR ←	DNA‑binding transcriptional repressor MngR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	765,189	0	T	.	100.0%	424.6 / NA	87	coding (687/723 nt)	mngR	DNA‑binding transcriptional repressor MngR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (52/35);  total (52/35)

GCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACACC  >  NC_000913/765140‑765234                                                  |                                              gCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAAt                                               >  1:75218/1‑50 (MQ=255) gCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAAt                                               >  1:3103345/1‑50 (MQ=255) gCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATAAGGGTAAAt                                               <  1:2038509/50‑1 (MQ=255)  ccTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAAtt                                              <  1:5475952/50‑1 (MQ=255)  ccTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAAtt                                              <  1:1321172/50‑1 (MQ=255)   cTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAAtta                                             >  1:5976723/1‑49 (MQ=255)   cTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAAtta                                             >  1:599834/1‑49 (MQ=255)    ttttttGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAAtta                                             >  1:5050840/1‑48 (MQ=255)    ttttttGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAAtta                                             >  1:4650141/1‑48 (MQ=255)     tttttGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAAtta                                             >  1:5692911/1‑47 (MQ=255)     tttttGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAAtta                                             >  1:2899518/1‑47 (MQ=255)      ttttGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAAttat                                            <  1:1607090/48‑3 (MQ=255)        ttGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGt                                        <  1:3115664/49‑1 (MQ=38)        ttGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGt                                        >  1:4232799/1‑49 (MQ=38)          gtgCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCAt                                     >  1:4466873/1‑50 (MQ=39)          gtgCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCAt                                     >  1:4868728/1‑50 (MQ=39)           tgCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCAt                                     <  1:5338718/49‑1 (MQ=38)           tgCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATc                                    <  1:1073501/50‑1 (MQ=39)           tgCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATc                                    >  1:1811825/1‑50 (MQ=39)           tgCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATc                                    >  1:645834/1‑50 (MQ=39)            gCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCg                                   >  1:3074232/1‑50 (MQ=39)             cTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCgg                                  <  1:5263983/50‑1 (MQ=39)             cTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCgg                                  >  1:3990505/1‑50 (MQ=39)             cTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCgg                                  >  1:3349886/1‑50 (MQ=39)             cTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCg                                   <  1:2919889/49‑1 (MQ=38)              ttATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCgg                                  >  1:4807806/1‑49 (MQ=38)                aTCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGt                                 <  1:3445080/48‑1 (MQ=38)                aTCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGt                                 <  1:5743926/48‑1 (MQ=38)                aTCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTAt                               >  1:2242416/1‑50 (MQ=39)                 tCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTAtt                              <  1:6326316/50‑1 (MQ=39)                 tCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTAt                               <  1:892563/49‑1 (MQ=38)                 tCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTAt                               >  1:6672582/1‑49 (MQ=38)                 tCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTAt                               <  1:3062599/49‑1 (MQ=38)                 gcgcgATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTAtt                              <  1:4804637/49‑1 (MQ=38)                  cgcgATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTA                             >  1:6281867‑M1/1‑50 (MQ=255)                   gcgATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTA                             >  1:3244604‑M1/1‑49 (MQ=255)                   gcgATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTA                             <  1:406596‑M1/49‑1 (MQ=255)                   gcgATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTA                             >  1:3682858‑M1/1‑49 (MQ=255)                   gcgATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTA                             <  1:6408918‑M1/49‑1 (MQ=255)                   gcgATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTA                             >  1:5439622‑M1/1‑49 (MQ=255)                    cgATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTA                             >  1:2862813‑M1/1‑48 (MQ=255)                     gatgatTTTCGCTGCGCTATCAGGGTACA‑TTATAGTCATCGGTATTAtag                          >  1:4731087‑M1/1‑47 (MQ=255)                     gatgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtag                          >  1:5266466‑M1/1‑47 (MQ=255)                     gatgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtag                          >  1:8470‑M1/1‑47 (MQ=255)                     gatgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtag                          >  1:6073672‑M1/1‑47 (MQ=255)                     gatgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtag                          >  1:6404332‑M1/1‑47 (MQ=255)                     gatgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtag                          >  1:4793564‑M1/1‑47 (MQ=255)                     gatgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtag                          <  1:473933‑M1/50‑4 (MQ=255)                     gatgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtag                          >  1:4705428‑M1/1‑47 (MQ=255)                     gatgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtag                          <  1:2344743‑M1/50‑4 (MQ=255)                     gatgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtag                          >  1:4623622‑M1/1‑47 (MQ=255)                     gatgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtag                          >  1:291201‑M1/1‑47 (MQ=255)                     gatgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtag                          >  1:3113578‑M1/1‑47 (MQ=255)                      atgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagc                         <  1:6034710‑M1/50‑5 (MQ=255)                      atgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtag                          >  1:5680114‑M1/1‑46 (MQ=255)                      atgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtag                          >  1:5860585‑M1/1‑46 (MQ=255)                      atgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtag                          >  1:2181828‑M1/1‑46 (MQ=255)                       tgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagca                        <  1:6081422‑M1/50‑6 (MQ=255)                       tgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagca                        <  1:3823492‑M1/50‑6 (MQ=255)                         atTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaat                      >  1:2525199‑M1/1‑43 (MQ=255)                          ttttCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatc                     <  1:4188734‑M1/50‑9 (MQ=255)                           tttCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatc                     >  1:649862‑M1/1‑41 (MQ=255)                           tttCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatc                     >  1:185297‑M1/1‑41 (MQ=255)                            ttCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatca                    >  1:1386927‑M1/1‑40 (MQ=255)                            ttCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatca                    <  1:3052243‑M1/49‑10 (MQ=255)                            ttCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatca                    >  1:1508816‑M1/1‑40 (MQ=255)                            ttCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatca                    >  1:3790011‑M1/1‑40 (MQ=255)                             tCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaa                  >  1:6170231‑M1/1‑39 (MQ=255)                              cGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaac                 >  1:726653‑M1/1‑38 (MQ=255)                              cGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaa                  <  1:589878‑M1/49‑12 (MQ=255)                              cGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaa                  <  1:1726051‑M1/49‑12 (MQ=255)                              cGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaa                  <  1:6413530‑M1/49‑12 (MQ=255)                               gCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaacc                >  1:1443838‑M1/1‑37 (MQ=255)                               gCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaacc                <  1:3778584‑M1/50‑14 (MQ=255)                               gCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaacc                >  1:3914333‑M1/1‑37 (MQ=255)                                cTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccg               <  1:6200750‑M1/50‑15 (MQ=255)                                cTGCGCTATCAAGGTAAA‑TTACAGTCATCGGTATTAtagcaatcaaaccg               <  1:4699686‑M1/50‑15 (MQ=255)                                 tGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccg               >  1:4774659‑M1/1‑35 (MQ=255)                                  gcgcTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccg               >  1:610337‑M1/1‑34 (MQ=255)                                  gcgcTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccg               >  1:3340618‑M1/1‑34 (MQ=255)                                   cgcTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaag            <  1:3816119‑M1/50‑18 (MQ=255)                                   cgcTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaag            >  1:5321858‑M1/1‑33 (MQ=255)                                   cgcTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccga              <  1:5096170‑M1/48‑16 (MQ=255)                                    gcTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagc           >  1:5371210‑M1/1‑32 (MQ=255)                                     cTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagc           >  1:3624153‑M1/1‑31 (MQ=255)                                      tATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagcca         >  1:2159568‑M1/1‑30 (MQ=255)                                       aTCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagcca         >  1:941302‑M1/1‑29 (MQ=255)                                       aTCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagcca         >  1:3457370‑M1/1‑29 (MQ=255)                                        tCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccaca       <  1:6340854‑M1/50‑23 (MQ=255)                                        tCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccaca       >  1:6318277‑M1/1‑28 (MQ=255)                                        tCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagcca         <  1:560328‑M1/48‑21 (MQ=255)                                        tCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagcca         <  1:3287325‑M1/48‑21 (MQ=255)                                         cAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccaca       >  1:400682‑M1/1‑27 (MQ=255)                                          aGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccacata     >  1:4067491‑M1/1‑26 (MQ=255)                                           gggTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccacatat    <  1:1803701‑M1/50‑26 (MQ=255)                                           gggTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccacatat    <  1:1668435‑M1/50‑26 (MQ=255)                                            ggTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccacatatg   <  1:5571976‑M1/50‑27 (MQ=255)                                             gTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccacatatgc  >  1:6002358‑M1/1‑23 (MQ=255)                                             gTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccacatatgc  <  1:116474‑M1/50‑28 (MQ=255)                                                  |                                              GCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACACC  >  NC_000913/765140‑765234

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑