BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,270,811	C→T	V347M (GTG→ATG)	chaA ←	Na(+)/K(+):H(+) antiporter ChaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,270,811	0	C	T	100.0%	482.1 / NA	117	V347M (GTG→ATG)	chaA	Na(+)/K(+):H(+) antiporter ChaA
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (45/72); total (45/72)

Only 100 of 117 total aligned reads displayed.

ATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAG  >  NC_000913/1270765‑1270857

atcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGt                                              <  1:528875/49‑1 (MQ=255)

atcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGt                                              <  1:1181633/49‑1 (MQ=255)

 tcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTa                                            <  1:6409074/50‑1 (MQ=255)

 tcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTa                                            <  1:4715589/50‑1 (MQ=255)

 tcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTa                                            <  1:4633402/50‑1 (MQ=255)

 tcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTa                                            <  1:1262508/50‑1 (MQ=255)

  caAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAg                                           >  1:1040097/1‑50 (MQ=255)

  caAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAg                                           >  1:2822854/1‑50 (MQ=255)

   aaaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAg                                           >  1:3924898/1‑49 (MQ=255)

   aaaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAg                                           >  1:245111/1‑49 (MQ=255)

    aaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTa                                         >  1:4712522/1‑50 (MQ=255)

     aTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTAc                                        <  1:4849573/50‑1 (MQ=255)

      tAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACg                                       <  1:1946409/50‑1 (MQ=255)

      tAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACg                                       >  1:4015196/1‑50 (MQ=255)

       aGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGt                                      >  1:6700367/1‑50 (MQ=255)

        ggcggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGt                                      <  1:4039358/49‑1 (MQ=255)

        ggcggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTc                                     <  1:5829826/50‑1 (MQ=255)

         gcggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTcc                                    <  1:4623382/50‑1 (MQ=255)

         gcggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTcc                                    <  1:5617570/50‑1 (MQ=255)

         gcggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTcc                                    <  1:5171049/50‑1 (MQ=255)

          cggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCg                                   <  1:3587198/50‑1 (MQ=255)

          cggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCg                                   <  1:5634479/50‑1 (MQ=255)

          cggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCg                                   <  1:2900851/50‑1 (MQ=255)

          cggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCg                                   <  1:58746/50‑1 (MQ=255)

           ggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCgg                                  <  1:708558/50‑1 (MQ=255)

           ggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCgg                                  >  1:78910/1‑50 (MQ=255)

           ggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCgg                                  <  1:436405/50‑1 (MQ=255)

            gcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGt                                 >  1:1338410/1‑50 (MQ=255)

            gcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGt                                 <  1:4979319/50‑1 (MQ=255)

             cAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCggag                                >  1:3630108/1‑48 (MQ=39)

             cAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg                                <  1:54039/50‑1 (MQ=255)

             cAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg                                <  1:1441891/50‑1 (MQ=255)

             cAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg                                <  1:6191940/50‑1 (MQ=255)

              aaaCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTgg                               <  1:2435517/50‑1 (MQ=255)

              aaaCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg                                >  1:5879622/1‑49 (MQ=255)

                aCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg                                >  1:1814054/1‑47 (MQ=255)

                aCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg                                >  1:461684/1‑47 (MQ=255)

                aCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGgag                             <  1:623554/50‑1 (MQ=255)

                 cAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGgaga                            <  1:1152653/50‑1 (MQ=255)

                 cAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGgag                             >  1:5440818/1‑49 (MQ=255)

                 cAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGgag                             >  1:1493181/1‑49 (MQ=255)

                  aGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGgag                             >  1:3040291/1‑48 (MQ=255)

                  aGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGgag                             >  1:2533372/1‑48 (MQ=255)

                  aGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGgag                             >  1:6078934/1‑48 (MQ=255)

                   gTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                          >  1:587059/1‑50 (MQ=255)

                    tGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAgg                         <  1:5447834/50‑1 (MQ=255)

                    tGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                          >  1:90892/1‑49 (MQ=255)

                     gCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                          >  1:4869762/1‑48 (MQ=255)

                      ccAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                          >  1:1874502/1‑47 (MQ=255)

                      ccAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGga                        <  1:4515092/49‑1 (MQ=255)

                      ccAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGga                        <  1:5627446/49‑1 (MQ=255)

                      ccAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGga                        <  1:382685/49‑1 (MQ=255)

                      ccAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGga                        <  1:593048/49‑1 (MQ=255)

                       cAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                          >  1:5066274/1‑46 (MQ=255)

                       cAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGgaga                      <  1:5503755/50‑1 (MQ=255)

                       cAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGgaga                      <  1:2958662/50‑1 (MQ=255)

                       cAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGgaga                      <  1:6168180/50‑1 (MQ=255)

                        aGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGat                     <  1:5952126/50‑1 (MQ=255)

                         gATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGata                    <  1:3878895/50‑1 (MQ=255)

                          aTGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGatat                   <  1:5000475/50‑1 (MQ=255)

                          aTGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGatat                   >  1:2380020/1‑50 (MQ=255)

                          aTGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGatat                   <  1:6593060/50‑1 (MQ=255)

                          aTGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGatat                   <  1:4512418/50‑1 (MQ=255)

                           tgcgctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATg                  <  1:974944/50‑1 (MQ=255)

                           tgcgctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATg                  >  1:1936249/1‑50 (MQ=255)

                            gcgctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATgg                 >  1:866443/1‑50 (MQ=255)

                             cgctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATgg                 >  1:374703/1‑49 (MQ=255)

                             cgctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATgg                 <  1:4257587/49‑1 (MQ=255)

                              gctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGca               >  1:5005769/1‑50 (MQ=255)

                              gctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGca               <  1:3740742/50‑1 (MQ=255)

                              gctgcgcCATTGAGCATGTTAGTACGTCCGGTGAAGAAGGAGATATGGca               <  1:190228/50‑1 (MQ=255)

                                tgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGca               >  1:873927/1‑48 (MQ=255)

                                tgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGca               >  1:5358143/1‑48 (MQ=255)

                                 gcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAg            >  1:2395934/1‑50 (MQ=255)

                                  cgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGcaca             <  1:5790836/48‑1 (MQ=255)

                                  cgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGcaca             <  1:6380066/48‑1 (MQ=255)

                                  cgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAg            >  1:1897645/1‑49 (MQ=255)

                                  cgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAg            <  1:5648398/49‑1 (MQ=255)

                                  cgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAg            >  1:5191546/1‑49 (MQ=255)

                                  cgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAg            <  1:3719662/49‑1 (MQ=255)

                                  cgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGc           <  1:6266665/50‑1 (MQ=255)

                                  cgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGc           <  1:488880/50‑1 (MQ=255)

                                    ccATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCAc         >  1:5715190/1‑50 (MQ=255)

                                     cATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCAc         >  1:2810094/1‑49 (MQ=255)

                                     cATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACt        <  1:6539866/50‑1 (MQ=255)

                                     cATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACt        <  1:1163474/50‑1 (MQ=255)

                                      aTTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACt        >  1:6079901/1‑49 (MQ=255)

                                      aTTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTa       <  1:1430123/50‑1 (MQ=255)

                                      aTTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTa       <  1:5234614/50‑1 (MQ=255)

                                      aTTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTa       <  1:1104942/50‑1 (MQ=255)

                                       ttGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTaa      <  1:2270776/50‑1 (MQ=255)

                                       ttGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTaa      <  1:1018355/50‑1 (MQ=255)

                                       ttGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTaa      <  1:249162/50‑1 (MQ=255)

                                        tGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTa       >  1:1469409/1‑48 (MQ=255)

                                         gAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTaaa     <  1:4390248/49‑1 (MQ=255)

                                         gAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTaaa     <  1:35976/49‑1 (MQ=255)

                                          aGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAaga   <  1:4224818/50‑1 (MQ=255)

                                           gCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAagag  >  1:5460957/1‑50 (MQ=255)

                                           gCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAagag  >  1:515664/1‑50 (MQ=255)

                                           gCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAagag  >  1:3551102/1‑50 (MQ=255)

ATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAG  >  NC_000913/1270765‑1270857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`