Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,382,555	C→T	S203S (AGC→AGT)	ompG →	outer membrane porin G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,382,555	0	C	T	100.0%	313.6 / NA	75	S203S (AGC→AGT)	ompG	outer membrane porin G
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/53);  total (22/53)

TTGCGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGCCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTGC  >  NC_000913/1382508‑1382601                                                |                                               ttGCGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCg                                              >  1:6476470/1‑50 (MQ=255) ttGCGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCg                                              >  1:2592089/1‑50 (MQ=255)   gCGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCg                                              <  1:3651377/48‑1 (MQ=255)    cGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCaa                                           <  1:3203199/50‑1 (MQ=255)    cGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCaa                                           <  1:6638005/50‑1 (MQ=255)    cGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCa                                            <  1:4985929/49‑1 (MQ=255)     gAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCaa                                           >  1:5110701/1‑49 (MQ=255)     gAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAAt                                          <  1:895167/50‑1 (MQ=255)     gAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAAt                                          <  1:766005/50‑1 (MQ=255)       gTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATaa                                        <  1:3721408/50‑1 (MQ=255)       gTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATaa                                        >  1:6165153/1‑50 (MQ=255)       gTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATaa                                        <  1:527452/50‑1 (MQ=255)        tGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAAc                                       <  1:741275/50‑1 (MQ=255)        tGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAAc                                       <  1:2350787/50‑1 (MQ=255)         gAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACg                                      >  1:6108484/1‑50 (MQ=255)          aaCTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACgg                                     >  1:1560183/1‑50 (MQ=255)          aaCTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACg                                      <  1:5260332/49‑1 (MQ=255)          aaCTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACg                                      <  1:6303386/49‑1 (MQ=255)          aaCTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACg                                      <  1:784073/49‑1 (MQ=255)           aCTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGt                                    <  1:4196733/50‑1 (MQ=255)            cTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTg                                   <  1:3317352/50‑1 (MQ=255)             tattatCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGt                                    >  1:5195643/1‑48 (MQ=255)               ttatCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGa                                  <  1:2641103/48‑1 (MQ=255)               ttatCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGa                                  <  1:760920/48‑1 (MQ=255)               ttatCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAg                                 <  1:2335338/49‑1 (MQ=255)               ttatCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGt                                >  1:271269/1‑50 (MQ=255)                 atCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGtt                               <  1:2967803/49‑1 (MQ=255)                  tctcGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGttt                              <  1:3585738/49‑1 (MQ=255)                    tcGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTc                            >  1:6686779/1‑49 (MQ=255)                     cGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCa                          >  1:2291820/1‑50 (MQ=255)                      gAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTcc                           <  1:4921458/48‑1 (MQ=255)                      gAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTcc                           <  1:2773281/48‑1 (MQ=255)                      gAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTcc                           <  1:5349515/48‑1 (MQ=255)                      gAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTc                            <  1:6651667/47‑1 (MQ=255)                      gAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTc                            <  1:1864625/47‑1 (MQ=255)                      gAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTc                            <  1:4671087/47‑1 (MQ=255)                      gAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCAc                         >  1:6511524/1‑50 (MQ=255)                      gAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCAc                         <  1:2249839/50‑1 (MQ=255)                        gcgcGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGc                       <  1:4324386/50‑1 (MQ=255)                         cgcgGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGc                       >  1:6428676/1‑49 (MQ=255)                         cgcgGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGc                       >  1:5201386/1‑49 (MQ=255)                         cgcgGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGc                       >  1:2388267/1‑49 (MQ=255)                          gcgGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCaa                     <  1:1623332/50‑1 (MQ=255)                           cgGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAg                    <  1:2568739/50‑1 (MQ=255)                            ggCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAg                    >  1:3233878/1‑49 (MQ=255)                            ggCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGa                   <  1:4354023/50‑1 (MQ=255)                            ggCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGa                   <  1:1656540/50‑1 (MQ=255)                                 cAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAAtt               <  1:3755632/49‑1 (MQ=255)                                 cAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAAtt               <  1:71967/49‑1 (MQ=255)                                 cAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAAt                <  1:1919691/48‑1 (MQ=255)                                 cAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAAt                <  1:2185529/48‑1 (MQ=255)                                 cAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAAt                <  1:5430013/48‑1 (MQ=255)                                  aaTATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTcg             >  1:2329711/1‑50 (MQ=255)                                  aaTATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTc              <  1:1745565/49‑1 (MQ=255)                                   atatGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTcgc            <  1:4399100/50‑1 (MQ=255)                                   atatGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTcgc            >  1:4847419/1‑50 (MQ=255)                                     atGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCgcgc          >  1:241536/1‑50 (MQ=255)                                      tGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCgcgc          <  1:86574/49‑1 (MQ=255)                                      tGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGcc         >  1:431407/1‑50 (MQ=255)                                      tGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGcc         <  1:3441134/50‑1 (MQ=255)                                      tGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGcc         <  1:4900837/50‑1 (MQ=255)                                       ggACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGcc         >  1:1627994/1‑49 (MQ=255)                                        gacgacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCt        <  1:1875975/49‑1 (MQ=255)                                        gacgacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTa       <  1:6315604/50‑1 (MQ=255)                                        gacgacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTa       >  1:4469190/1‑50 (MQ=255)                                         acgacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTAt      <  1:3212262/50‑1 (MQ=255)                                         acgacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTAt      <  1:3823392/50‑1 (MQ=255)                                          cgacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTAtt     <  1:2667926/50‑1 (MQ=255)                                          cgacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTAtt     <  1:6256260/50‑1 (MQ=255)                                          cgacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTAtt     <  1:5264361/50‑1 (MQ=255)                                          cgacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTAtt     <  1:3883751/50‑1 (MQ=255)                                          cgacAGTCGCAATAACGGTGAGTTTTCCAAGCAAGAAATTCGCGCCTAtt     <  1:5943842/50‑1 (MQ=255)                                           gacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTAttt    >  1:4237713/1‑50 (MQ=255)                                            acAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTg   <  1:3861575/50‑1 (MQ=255)                                             cAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTGc  <  1:3331129/50‑1 (MQ=255)                                                |                                               TTGCGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGCCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTGC  >  NC_000913/1382508‑1382601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑