Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,742,755	G→C	P163R (CCG→CGG)	ribC ←	riboflavin synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,742,755	0	G	C	100.0%	398.8 / NA	96	P163R (CCG→CGG)	ribC	riboflavin synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (57/39);  total (57/39)

GTGCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCGGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACGG  >  NC_000913/1742708‑1742800                                                |                                              gTGCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGa                                             <  1:1743845/50‑1 (MQ=255)  tGCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGaa                                            >  1:2850689/1‑50 (MQ=255)    cgcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAAtt                                          <  1:2494562/50‑1 (MQ=255)    cgcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAAt                                           >  1:916497/1‑49 (MQ=255)    cgcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAAt                                           <  1:310719/49‑1 (MQ=255)     gcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAAtt                                          >  1:45086/1‑49 (MQ=255)     gcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAAtt                                          >  1:1344926/1‑49 (MQ=255)     gcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAAtt                                          >  1:6534314/1‑49 (MQ=255)     gcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAAtt                                          >  1:1739237/1‑49 (MQ=255)      ccAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTaa                                        <  1:810629/50‑1 (MQ=255)      ccAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTaa                                        <  1:4353139/50‑1 (MQ=255)      ccAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTaa                                        <  1:3343590/50‑1 (MQ=255)      ccAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTa                                         >  1:4800266/1‑49 (MQ=255)      ccAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTa                                         >  1:3486330/1‑49 (MQ=255)      ccAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTa                                         >  1:647566/1‑49 (MQ=255)      ccAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTa                                         >  1:1767003/1‑49 (MQ=255)       cAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTaaa                                       >  1:2946836/1‑50 (MQ=255)       cAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTaaa                                       >  1:1100483/1‑50 (MQ=255)        aaGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAAt                                      <  1:5136378/50‑1 (MQ=255)        aaGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAAt                                      <  1:5561371/50‑1 (MQ=255)        aaGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAAt                                      <  1:2322301/50‑1 (MQ=255)        aaGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAAt                                      >  1:6392726/1‑50 (MQ=255)         aGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATg                                     <  1:2016464/50‑1 (MQ=255)         aGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATg                                     <  1:1984688/50‑1 (MQ=255)          gTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATgg                                    <  1:2623170/50‑1 (MQ=255)          gTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATg                                     >  1:4117531/1‑49 (MQ=255)          gTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATg                                     >  1:2770022/1‑49 (MQ=255)          gTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATg                                     >  1:3465619/1‑49 (MQ=255)             ttttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGACTTAAATGGACg                                 >  1:5377571/1‑50 (MQ=255)             ttttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGAc                                  <  1:325225/49‑1 (MQ=255)             ttttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACg                                 >  1:2676661/1‑50 (MQ=255)             ttttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACg                                 >  1:2218532/1‑50 (MQ=255)              tttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACg                                 >  1:6085284/1‑49 (MQ=255)              tttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACg                                 >  1:5851729/1‑49 (MQ=255)              tttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACg                                 >  1:6039789/1‑49 (MQ=255)               ttcttcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGc                                <  1:3873715/49‑1 (MQ=255)               ttcttcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGc                                >  1:4420382/1‑49 (MQ=255)               ttcttcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGc                                <  1:2847454/49‑1 (MQ=255)                   tcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaaaaa                           >  1:4480186/1‑50 (MQ=255)                   tcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaaaaa                           >  1:4987827/1‑50 (MQ=255)                    cccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaaaaa                           <  1:535606/49‑1 (MQ=255)                    cccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaaa                             <  1:472706/47‑1 (MQ=255)                    cccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaaa                             <  1:5596299/47‑1 (MQ=255)                     ccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAcg                         <  1:4422387/50‑1 (MQ=255)                     ccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAcg                         <  1:2754125/50‑1 (MQ=255)                     ccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAc                          <  1:6257278/49‑1 (MQ=255)                     ccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAc                          <  1:3499858/49‑1 (MQ=255)                      cAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAcgc                        <  1:6471388/50‑1 (MQ=255)                      cAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAcgc                        <  1:5684684/50‑1 (MQ=255)                       aaGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAcgcg                       >  1:3558794/1‑50 (MQ=255)                       aaGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAcgcg                       >  1:5001950/1‑50 (MQ=255)                       aaGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAcgcg                       <  1:6040444/50‑1 (MQ=255)                        agagTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGt                      <  1:5182825/50‑1 (MQ=255)                        agagTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGt                      >  1:5224721/1‑50 (MQ=255)                         gagTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGtt                     <  1:3895806/50‑1 (MQ=255)                         gagTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGtt                     <  1:4578198/50‑1 (MQ=255)                         gagTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGtt                     >  1:4914870/1‑50 (MQ=255)                          agTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTg                    <  1:5463809/50‑1 (MQ=255)                          agTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTg                    >  1:1874949/1‑50 (MQ=255)                           gTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTgg                   <  1:5788175/50‑1 (MQ=255)                           gTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTgg                   >  1:3927883/1‑50 (MQ=255)                           gTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTgg                   <  1:2368438/50‑1 (MQ=255)                            tCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGc                  <  1:1759462/50‑1 (MQ=255)                            tCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGc                  <  1:3148886/50‑1 (MQ=255)                             cGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCg                 >  1:462266/1‑50 (MQ=255)                              gTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGt                >  1:4602128/1‑50 (MQ=255)                              gTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGt                >  1:1076707/1‑50 (MQ=255)                              gTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGt                >  1:3689535/1‑50 (MQ=255)                              gTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGt                >  1:5235227/1‑50 (MQ=255)                               tGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGt                >  1:3027190/1‑49 (MQ=255)                               tGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTg               <  1:1736490/50‑1 (MQ=255)                                gCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGa              >  1:6095888/1‑50 (MQ=255)                                gCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGa              >  1:3195931/1‑50 (MQ=255)                                 cGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGAc             >  1:2715054/1‑50 (MQ=255)                                 cGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGAc             >  1:2255731/1‑50 (MQ=255)                                  gTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACt            >  1:1891069/1‑50 (MQ=255)                                  gTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACt            >  1:1275599/1‑50 (MQ=255)                                  gTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACt            >  1:988982/1‑50 (MQ=255)                                  gTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACt            >  1:3009248/1‑50 (MQ=255)                                   ttCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACtt           >  1:3553540/1‑50 (MQ=255)                                   ttCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACtt           >  1:4863640/1‑50 (MQ=255)                                   ttCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACtt           >  1:5293742/1‑50 (MQ=255)                                     ccAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTc          <  1:5736390/49‑1 (MQ=255)                                     ccAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCg         >  1:2401707/1‑50 (MQ=255)                                      cAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGc        >  1:486090/1‑50 (MQ=255)                                      cAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGc        <  1:1346271/50‑1 (MQ=255)                                       aGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGcc       >  1:2257056/1‑50 (MQ=255)                                       aGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGcc       <  1:5530206/50‑1 (MQ=255)                                       aGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGcc       <  1:2072859/50‑1 (MQ=255)                                       aGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGcc       >  1:185629/1‑50 (MQ=255)                                       aGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGcc       >  1:3729941/1‑50 (MQ=255)                                         tgtTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGa     >  1:5590029/1‑50 (MQ=255)                                         tgtTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGa     >  1:5974244/1‑50 (MQ=255)                                          gtTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGAc    >  1:5185118/1‑50 (MQ=255)                                           tttCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGAc    <  1:2173282/49‑1 (MQ=255)                                            ttCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACgg  >  1:4173051/1‑50 (MQ=255)                                                |                                              GTGCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCGGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACGG  >  NC_000913/1742708‑1742800

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑