BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	257,794	T→C	intergenic (+23/‑35)	frsA → / → crl	fermentation‑respiration switch protein/RNA polymerase holoenzyme assembly factor Crl

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	257,794	0	T	C	100.0%	482.1 / NA	114	intergenic (+23/‑35)	frsA/crl	fermentation‑respiration switch protein/RNA polymerase holoenzyme assembly factor Crl
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (53/61); total (53/61)

Only 100 of 114 total aligned reads displayed.

CTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCG  >  NC_000913/257747‑257840

cTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTg                                              >  1:1422606/1‑50 (MQ=255)

cTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTg                                              <  1:3544758/50‑1 (MQ=255)

 tGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTgg                                             >  1:264527/1‑50 (MQ=255)

   gATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTgg                                             <  1:2530378/48‑1 (MQ=255)

   gATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTgg                                             <  1:3685872/48‑1 (MQ=255)

   gATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGt                                            >  1:2278033/1‑49 (MQ=255)

   gATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGt                                            >  1:2035268/1‑49 (MQ=255)

    aTCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaa                                          <  1:2860388/50‑1 (MQ=255)

    aTCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTa                                           >  1:1894317/1‑49 (MQ=255)

     tCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaaa                                         <  1:4031484/50‑1 (MQ=255)

     tCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaaa                                         <  1:2007411/50‑1 (MQ=255)

     tCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaaa                                         <  1:2683232/50‑1 (MQ=255)

     tCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaa                                          >  1:4011595/1‑49 (MQ=255)

     tCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaa                                          >  1:625171/1‑49 (MQ=255)

       gAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaaaa                                        >  1:5194281/1‑49 (MQ=255)

       gAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaaaa                                        >  1:3719745/1‑49 (MQ=255)

       gAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaaaa                                        >  1:4746054/1‑49 (MQ=255)

       gAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaaaa                                        <  1:4316465/49‑1 (MQ=255)

       gAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaaaa                                        <  1:1699829/49‑1 (MQ=255)

       gAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAAc                                       <  1:372049/50‑1 (MQ=255)

       gAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAAc                                       <  1:3174125/50‑1 (MQ=255)

        aaaaaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaaaa                                        >  1:1445395/1‑48 (MQ=255)

        aaaaaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaaaa                                        >  1:1812881/1‑48 (MQ=255)

         aaaaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAg                                     >  1:5167716/1‑50 (MQ=255)

          aaaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGt                                    <  1:6529185/50‑1 (MQ=255)

          aaaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGt                                    <  1:5174015/50‑1 (MQ=255)

           aaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGtt                                   <  1:4152138/50‑1 (MQ=255)

           aaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGtt                                   <  1:3656668/50‑1 (MQ=255)

            aCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTg                                  >  1:2676199/1‑50 (MQ=255)

            aCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTg                                  <  1:3372221/50‑1 (MQ=255)

              gCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCa                                <  1:683762/50‑1 (MQ=255)

              gCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCa                                <  1:4319373/50‑1 (MQ=255)

              gCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCa                                >  1:4910405/1‑50 (MQ=255)

               cTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCAt                               >  1:5654083/1‑50 (MQ=255)

               cTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCAt                               <  1:5703792/50‑1 (MQ=255)

                ttGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCAt                               >  1:481497/1‑49 (MQ=255)

                ttGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCAt                               >  1:4454339/1‑49 (MQ=255)

                  gtgtTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATca                             <  1:6280658/49‑1 (MQ=255)

                  gtgtTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATca                             <  1:4846172/49‑1 (MQ=255)

                  gtgtTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATca                             >  1:5917514/1‑49 (MQ=255)

                   tgtTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATcac                            <  1:698734/49‑1 (MQ=255)

                     ttAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCacaac                         <  1:430365/50‑1 (MQ=255)

                     ttAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCacaa                          <  1:6665929/49‑1 (MQ=255)

                     ttAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCacaa                          >  1:6652629/1‑49 (MQ=255)

                     ttAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCacaa                          <  1:2316270/49‑1 (MQ=255)

                      tAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCacaa                          >  1:4334054/1‑48 (MQ=255)

                       aaaaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCacaaca                        <  1:6493075/49‑1 (MQ=255)

                       aaaaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCacaaca                        <  1:1787999/49‑1 (MQ=255)

                        aaaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAg                       <  1:1551288/49‑1 (MQ=255)

                        aaaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAg                       >  1:85210/1‑49 (MQ=255)

                        aaaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAg                       <  1:3158548/49‑1 (MQ=255)

                          aaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGgag                    <  1:4804657/50‑1 (MQ=255)

                          aaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGgag                    <  1:4183865/50‑1 (MQ=255)

                           aTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAg                       >  1:2514859/1‑46 (MQ=255)

                           aTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGgaga                   <  1:4092882/50‑1 (MQ=255)

                           aTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGgaga                   <  1:1091493/50‑1 (MQ=255)

                           aTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGgaga                   <  1:3719441/50‑1 (MQ=255)

                            tttGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGgaga                   >  1:1134217/1‑49 (MQ=255)

                            tttGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGgaga                   >  1:6276996/1‑49 (MQ=255)

                            tttGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGgaga                   >  1:5049967/1‑49 (MQ=255)

                            tttGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGAt                  <  1:4754425/50‑1 (MQ=255)

                             ttGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGgaga                   >  1:3443818/1‑48 (MQ=255)

                              tGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATa                 <  1:3016230/49‑1 (MQ=255)

                              tGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAg                >  1:1181150/1‑50 (MQ=255)

                              tGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAg                >  1:2256547/1‑50 (MQ=255)

                               gCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAg                >  1:3892568/1‑49 (MQ=255)

                               gCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAg                <  1:4673232/49‑1 (MQ=255)

                                cTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGc               <  1:4880741/49‑1 (MQ=255)

                                cTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGc               <  1:4900298/49‑1 (MQ=255)

                                cTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGc               <  1:2467072/49‑1 (MQ=255)

                                 tAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCaa             >  1:6480503/1‑50 (MQ=255)

                                  aaaTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAAt            >  1:6003794/1‑50 (MQ=255)

                                   aaTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAAt            <  1:1377958/49‑1 (MQ=255)

                                   aaTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAAt            <  1:3604804/49‑1 (MQ=255)

                                   aaTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATg           <  1:3739264/50‑1 (MQ=255)

                                   aaTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATg           <  1:3023586/50‑1 (MQ=255)

                                    aTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGa          <  1:4937040/50‑1 (MQ=255)

                                    aTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGa          >  1:5271304/1‑50 (MQ=255)

                                    aTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGa          >  1:5321941/1‑50 (MQ=255)

                                    aTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGa          >  1:4061069/1‑50 (MQ=255)

                                     ttttGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGAc         >  1:6210405/1‑50 (MQ=255)

                                     ttttGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGAc         >  1:4455450/1‑50 (MQ=255)

                                       ttGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACg        <  1:5618595/49‑1 (MQ=255)

                                       ttGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACg        <  1:2777447/49‑1 (MQ=255)

                                       ttGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACg        <  1:5533248/49‑1 (MQ=255)

                                         gCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGtt      <  1:3974441/49‑1 (MQ=255)

                                         gCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTa     >  1:4380902/1‑50 (MQ=255)

                                          ccAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTa     <  1:5953975/49‑1 (MQ=255)

                                          ccAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTa     <  1:761776/49‑1 (MQ=255)

                                          ccAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTAc    >  1:5167028/1‑50 (MQ=255)

                                          ccAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTAc    <  1:1397211/50‑1 (MQ=255)

                                          ccAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTAc    <  1:5043425/50‑1 (MQ=255)

                                          ccAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTAc    >  1:4639561/1‑50 (MQ=255)

                                           cAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTAcc   >  1:3208431/1‑50 (MQ=255)

                                            aaTCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTAcc   >  1:6183536/1‑49 (MQ=255)

                                            aaTCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTAcc   >  1:5625691/1‑49 (MQ=255)

                                            aaTCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTAcc   >  1:331614/1‑49 (MQ=255)

                                            aaTCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTAcc   >  1:2045387/1‑49 (MQ=255)

                                            aaTCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTAcc   >  1:4598365/1‑49 (MQ=255)

                                            aaTCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCg  <  1:2461471/50‑1 (MQ=255)

CTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCG  >  NC_000913/257747‑257840

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`