BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,477,150	C→T	K90K (AAG→AAA)	fkpA ←	peptidyl‑prolyl cis‑trans isomerase FkpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,477,150	0	C	T	100.0%	455.6 / NA	111	K90K (AAG→AAA)	fkpA	peptidyl‑prolyl cis‑trans isomerase FkpA
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (46/65); total (46/65)

Only 100 of 114 total aligned reads displayed.

CGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTA  >  NC_000913/3477104‑3477195

cGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCtttt                                             <  1:4732636/49‑1 (MQ=255)

cGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCtttt                                             <  1:5180072/49‑1 (MQ=255)

cGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCtttt                                             <  1:143509/49‑1 (MQ=255)

cGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTa                                            >  1:6363020/1‑50 (MQ=255)

 gAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTa                                            <  1:2600324/49‑1 (MQ=255)

 gAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTAt                                           <  1:2119789/50‑1 (MQ=255)

 gAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTAt                                           >  1:4582775/1‑50 (MQ=255)

  aaTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTAt                                           >  1:2877466/1‑49 (MQ=255)

  aaTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTAt                                           >  1:2019602/1‑49 (MQ=255)

  aaTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATc                                          <  1:3589543/50‑1 (MQ=255)

  aaTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATc                                          <  1:609460/50‑1 (MQ=255)

   aTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTAt                                           >  1:1753295/1‑48 (MQ=255)

   aTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTAt                                           >  1:263488/1‑48 (MQ=255)

   aTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCa                                         <  1:714263/50‑1 (MQ=255)

    tGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAg                                        <  1:2555086/50‑1 (MQ=255)

      cTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGc                                       <  1:6445437/49‑1 (MQ=255)

      cTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCa                                      <  1:2528909/50‑1 (MQ=255)

        tGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCaaa                                    >  1:3029790/1‑50 (MQ=255)

        tGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCaaa                                    >  1:1072474/1‑50 (MQ=255)

         gTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCaaa                                    <  1:554435/49‑1 (MQ=255)

         gTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCaaa                                    <  1:2214870/49‑1 (MQ=255)

         gTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCaaa                                    <  1:2593897/49‑1 (MQ=255)

         gTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAAt                                   >  1:1001344/1‑50 (MQ=255)

         gTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAAt                                   >  1:1983832/1‑50 (MQ=255)

          tAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATg                                  <  1:5503584/50‑1 (MQ=255)

            gagTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGc                                 <  1:1838470/49‑1 (MQ=255)

            gagTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCa                                >  1:5011130/1‑50 (MQ=255)

            gagTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCa                                <  1:5052321/50‑1 (MQ=255)

            gagTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCa                                >  1:3518468/1‑50 (MQ=255)

            gagTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCa                                >  1:1832614/1‑50 (MQ=255)

             agTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCAt                               >  1:4336086/1‑50 (MQ=255)

             agTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCAt                               <  1:5446261/50‑1 (MQ=255)

              gTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATc                              <  1:2038262/50‑1 (MQ=255)

              gTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATc                              >  1:350315/1‑50 (MQ=255)

                 tGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCt                            >  1:3195273/1‑49 (MQ=255)

                  gTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTg                           >  1:1238476/1‑49 (MQ=255)

                  gTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTg                           >  1:2906890/1‑49 (MQ=255)

                   ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCt                            <  1:1056677/47‑1 (MQ=255)

                   ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTg                           <  1:2569427/48‑1 (MQ=255)

                   ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTg                           <  1:2284919/48‑1 (MQ=255)

                   ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGaa                         <  1:1336486/50‑1 (MQ=255)

                   ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGaa                         <  1:4990543/50‑1 (MQ=255)

                   ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGaa                         <  1:1438337/50‑1 (MQ=255)

                   ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGaa                         >  1:2883361/1‑50 (MQ=255)

                   ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGa                          <  1:1450641/49‑1 (MQ=255)

                   ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGa                          <  1:1478324/49‑1 (MQ=255)

                     cGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAaca                       >  1:6450407/1‑50 (MQ=255)

                     cGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAaca                       >  1:2959298/1‑50 (MQ=255)

                     cGATCTCTTGGTCGGACAGTTTGCTTTTATCAGCAAATGCATCCTGAac                        <  1:4208315/49‑1 (MQ=39)

                      gATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAacac                      >  1:1846307/1‑50 (MQ=255)

                      gATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAaca                       <  1:6599840/49‑1 (MQ=255)

                        tctcTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAacac                      >  1:1606496/1‑48 (MQ=255)

                          tctTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGc                  >  1:4811290/1‑50 (MQ=255)

                          tctTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGc                  >  1:2354938/1‑50 (MQ=255)

                          tctTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGc                  >  1:3658589/1‑50 (MQ=255)

                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACAcc                     <  1:4925942/45‑1 (MQ=255)

                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACAcc                     <  1:2231631/45‑1 (MQ=255)

                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCa                    <  1:2925160/46‑1 (MQ=255)

                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCa                    <  1:5478910/46‑1 (MQ=255)

                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCa                    <  1:3102438/46‑1 (MQ=255)

                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGc                  <  1:3431329/48‑1 (MQ=255)

                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGc                  <  1:5059149/48‑1 (MQ=255)

                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGa                >  1:793331/1‑50 (MQ=255)

                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGa                >  1:5526913/1‑50 (MQ=255)

                              ggTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCg                 <  1:3130522/47‑1 (MQ=255)

                              ggTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGAt               <  1:3620828/49‑1 (MQ=255)

                              ggTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGAt               <  1:3962271/49‑1 (MQ=255)

                              ggTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGAt               <  1:3277609/49‑1 (MQ=255)

                              ggTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGAt               <  1:6082209/49‑1 (MQ=255)

                               gTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATc              <  1:1509593/49‑1 (MQ=255)

                               gTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCa             <  1:2210431/50‑1 (MQ=255)

                               gTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCa             <  1:3174201/50‑1 (MQ=255)

                               gTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCa             <  1:304256/50‑1 (MQ=255)

                                tCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAg            <  1:1050147/50‑1 (MQ=255)

                                tCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAg            <  1:1261793/50‑1 (MQ=255)

                                 cGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGc           >  1:5288778/1‑50 (MQ=255)

                                 cGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGc           >  1:6010453/1‑50 (MQ=255)

                                 cGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGc           >  1:5922645/1‑50 (MQ=255)

                                  ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGc           <  1:4736098/49‑1 (MQ=255)

                                  ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCt          <  1:4772141/50‑1 (MQ=255)

                                  ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCt          >  1:2226378/1‑50 (MQ=255)

                                  ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCt          >  1:2970837/1‑50 (MQ=255)

                                  ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCt          >  1:5929706/1‑50 (MQ=255)

                                  ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCt          <  1:3936746/50‑1 (MQ=255)

                                  ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCt          >  1:4823403/1‑50 (MQ=255)

                                  ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCt          <  1:4675240/50‑1 (MQ=255)

                                   gagaGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTg         <  1:4041911/50‑1 (MQ=255)

                                   gagaGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTg         <  1:2680574/50‑1 (MQ=255)

                                    agagTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGa        >  1:1239122/1‑50 (MQ=255)

                                    agagTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGa        <  1:6541218/50‑1 (MQ=255)

                                     gagTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGAt       <  1:3565221/50‑1 (MQ=255)

                                     gagTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGAt       <  1:5716277/50‑1 (MQ=255)

                                     gagTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGAt       >  1:3135758/1‑50 (MQ=255)

                                      agTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATc      >  1:79867/1‑50 (MQ=255)

                                       gTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCt     <  1:567430/50‑1 (MQ=255)

                                       gTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCt     <  1:28005/50‑1 (MQ=255)

                                       gTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCt     <  1:4375944/50‑1 (MQ=255)

                                         ttGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCtt    >  1:1983095/1‑49 (MQ=255)

                                          tGCTTTTATCAGCAAATGCATCCTGAACACTAGCGATCAACTGCTCTTTa  >  1:4293852/1‑50 (MQ=255)

                                          tGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCttt   <  1:6097723/49‑1 (MQ=255)

CGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTA  >  NC_000913/3477104‑3477195

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`