Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,485,906	C→A	A16S (GCC→TCC)	pepA ←	aminopeptidase A/I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,485,906	0	C	A	100.0%	379.9 / NA	91	A16S (GCC→TCC)	pepA	aminopeptidase A/I
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (49/42);  total (49/42)

AATCGGAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCAT  >  NC_000913/4485859‑4485951                                                |                                              aaTCGGAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAAc                                             >  1:3281067/1‑50 (MQ=255) aaTCGGAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAAc                                             >  1:2124286/1‑50 (MQ=255) aaTCGGAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAAc                                             <  1:1746302/50‑1 (MQ=255)  aTCGGAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACt                                            >  1:4475971/1‑50 (MQ=255)  aTCGGAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACt                                            >  1:2329322/1‑50 (MQ=255)     ggAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCg                                         >  1:3526330/1‑50 (MQ=255)     ggAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCg                                         >  1:1744006/1‑50 (MQ=255)     ggAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCg                                         >  1:156736/1‑50 (MQ=255)     ggAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCg                                         >  1:5515394/1‑50 (MQ=255)      gagaAAGGCGACGTGGTTCGAAGACG‑‑CACGACGATGCAGGAACTCCGCTg                                      >  1:5678652/1‑50 (MQ=255)       agaAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCt                                       >  1:5967846/1‑50 (MQ=255)       agaAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCt                                       >  1:4936531/1‑50 (MQ=255)       agaAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCt                                       <  1:77691/50‑1 (MQ=255)         aaaGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGt                                     >  1:5634418/1‑50 (MQ=255)         aaaGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGt                                     <  1:2411424/50‑1 (MQ=255)         aaaGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGt                                     >  1:1131101/1‑50 (MQ=255)         aaaGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGt                                     <  1:2184426/50‑1 (MQ=255)           aGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGttt                                   >  1:4407118/1‑50 (MQ=255)            ggCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTtc                                  <  1:6094622/50‑1 (MQ=255)            ggCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTtc                                  <  1:2569124/50‑1 (MQ=255)             gCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTtct                                 <  1:4233575/50‑1 (MQ=255)             gCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTtct                                 <  1:6380023/50‑1 (MQ=255)             gCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTtct                                 <  1:1477705/50‑1 (MQ=255)              cGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTtctc                                <  1:291205/50‑1 (MQ=255)               gACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTcc                               <  1:1804433/50‑1 (MQ=255)               gACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTcc                               <  1:6148959/50‑1 (MQ=255)               gACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTcc                               <  1:681744/50‑1 (MQ=255)               gACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTcc                               <  1:4365199/50‑1 (MQ=255)                aCGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCg                              >  1:5429987/1‑50 (MQ=255)                 cGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCgg                             <  1:3257508/50‑1 (MQ=255)                 cGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCgg                             <  1:4800483/50‑1 (MQ=255)                  gTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCggg                            >  1:1424002/1‑50 (MQ=255)                  gTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCggg                            >  1:6276839/1‑50 (MQ=255)                  gTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCggg                            <  1:4724345/50‑1 (MQ=255)                   tGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGc                           >  1:3069196/1‑50 (MQ=255)                    ggTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGc                           >  1:1448599/1‑49 (MQ=255)                     gTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTa                         >  1:2393384/1‑50 (MQ=255)                     gTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTa                         <  1:5381464/50‑1 (MQ=255)                       tCGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTAcc                       >  1:71857/1‑50 (MQ=255)                        cGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTAcc                       <  1:915504/49‑1 (MQ=255)                        cGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTAcc                       <  1:13629/49‑1 (MQ=255)                        cGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTAcc                       <  1:741579/49‑1 (MQ=255)                        cGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTACCg                      >  1:3271903/1‑50 (MQ=255)                         gAAGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGc                     >  1:2369542/1‑50 (MQ=255)                          aaGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCt                    <  1:1269022/50‑1 (MQ=255)                          aaGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCt                    <  1:4924424/50‑1 (MQ=255)                          aaGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCt                    >  1:3705208/1‑50 (MQ=255)                           aGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCtt                   <  1:3052117/50‑1 (MQ=255)                           aGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCtt                   <  1:2443198/50‑1 (MQ=255)                           aGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCtt                   >  1:4496197/1‑50 (MQ=255)                           aGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCtt                   >  1:2328096/1‑50 (MQ=255)                           aGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCtt                   >  1:3796794/1‑50 (MQ=255)                           aGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCtt                   <  1:3577623/50‑1 (MQ=255)                           aGACGCCCACGACGATGCAGGAACTCCACTGTTTCTCCGGGCTACCGCtt                   <  1:6287906/50‑1 (MQ=255)                            gACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCttt                  <  1:1237608/50‑1 (MQ=255)                            gACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCttt                  <  1:5767288/50‑1 (MQ=255)                            gACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCttt                  <  1:1320399/50‑1 (MQ=255)                             aCGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCtttt                 <  1:548689/50‑1 (MQ=255)                             aCGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCtttt                 <  1:4250431/50‑1 (MQ=255)                             aCGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCtttt                 >  1:6146779/1‑50 (MQ=255)                               gCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTa               >  1:5223644/1‑50 (MQ=255)                               gCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTa               >  1:5408217/1‑50 (MQ=255)                                 ccACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTaca             <  1:5556649/50‑1 (MQ=255)                                 ccACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTaca             <  1:1127626/50‑1 (MQ=255)                                 ccACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTaca             >  1:1410324/1‑50 (MQ=255)                                  cACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTacac            <  1:644623/50‑1 (MQ=255)                                  cACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTacac            <  1:5230045/50‑1 (MQ=255)                                  cACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTacac            >  1:6594421/1‑50 (MQ=255)                                  cACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTacac            >  1:3181239/1‑50 (MQ=255)                                   acgacgATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTacac            >  1:5250816/1‑49 (MQ=255)                                   acgacgATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTacac            >  1:5573824/1‑49 (MQ=255)                                   acgacgATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTacac            >  1:1842830/1‑49 (MQ=255)                                   acgacgATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTacac            >  1:1237287/1‑49 (MQ=255)                                   acgacgATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACt           <  1:4776680/50‑1 (MQ=255)                                     gacgaTGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTa          >  1:3584144/1‑49 (MQ=255)                                     gacgaTGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTa          >  1:4620111/1‑49 (MQ=255)                                      acgaTGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTaaa        >  1:5926160/1‑50 (MQ=255)                                      acgaTGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTaaa        >  1:545957/1‑50 (MQ=255)                                       cgaTGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAAc       >  1:1764697/1‑50 (MQ=255)                                        gaTGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACt      <  1:1438316/50‑1 (MQ=255)                                        gaTGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACt      >  1:5452901/1‑50 (MQ=255)                                        gaTGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACt      >  1:67179/1‑50 (MQ=255)                                        gaTGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACt      <  1:2351735/50‑1 (MQ=255)                                        gaTGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACt      >  1:3285481/1‑50 (MQ=255)                                        gaTGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACt      <  1:3481006/50‑1 (MQ=255)                                         aTGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTc     <  1:2087377/50‑1 (MQ=255)                                          tGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTc     >  1:4223021/1‑49 (MQ=255)                                           gCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCa   >  1:6312769/1‑50 (MQ=255)                                            cAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCAt  >  1:65829/1‑50 (MQ=255)                                            cAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCAt  >  1:108533/1‑50 (MQ=255)                                            cAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCAt  <  1:997577/50‑1 (MQ=255)                                                |                                              AATCGGAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCAT  >  NC_000913/4485859‑4485951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑