| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,815,810 | Δ1 bp | intergenic (‑42/+53) | pyrE ← / ← rph | orotate phosphoribosyltransferase/truncated RNase PH |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,815,809 | 0 | G | . | 85.7% | 11.1 / ‑3.9 | 7 | intergenic (‑41/+54) | pyrE/rph | orotate phosphoribosyltransferase/truncated RNase PH |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base . (3/3); minor base C (1/0); total (4/3) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01 | |||||||||||
GCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGC > NC_000913/3815696‑3815911 | gCGTAAACTCGCCAAACTTTAACACCGGCTTGCTAAGCCCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGc < 1:202898/129‑1 (MQ=255) gCCAAAATTTAAACCCTGTTGTCCAAATGCAAATTCAATAAACTGGCGCTTAAATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATc < 1:158625/129‑1 (MQ=255) aaaCTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCGCATCTTACTTTTCTACAGCCAAAAAAAA‑GCGACTCATACGTCGCCTTAAAAATCa > 1:344486/1‑127 (MQ=255) aaaCTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTTTGCGTCGTTCCAAGGgatt > 1:189521/1‑129 (MQ=255) cgcTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGCCAAAAAAAACG‑GCCTCATCAGTCCCCTTAAAAA‑CAGTTTTCCCACGCCCCCCTCTTGGTCGCTACAAAGGATTCGATTccc > 1:136029/1‑129 (MQ=255) ccctcAACTTTCTTTTCCAACGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGa < 1:162811/128‑1 (MQ=255) aTCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTCAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCCCGGGCCAGAGCCCAAAAAATGAGTAGc > 1:97067/1‑129 (MQ=255) | GCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGC > NC_000913/3815696‑3815911 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |