Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,106,741	A→T	S162S (TCT→TCA)	wbbH ←	putative O‑antigen polymerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,106,741	0	A	T	100.0%	12.3 / NA	6	S162S (TCT→TCA)	wbbH	putative O‑antigen polymerase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (5/1);  total (5/1)

TGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTAGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAA  >  NC_000913/2106629‑2106854                                                                                                                 |                                                                                                                  tgatAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATCCATTAAAACAAAGTAAGTCAAATG‑TTTACTTTCCTTTGGTTTGAGAAATTTTTTTTCCCAaaaaaaaaaaaa                                                                                                  >  1:294323/1‑127 (MQ=255)               accaTTTGCTTAACCGTAATAAGTATAATGTCAAAGTTGAATACAATAAAACCAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGa                                                                                     <  1:344974/126‑1 (MQ=255)               aCAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGa                                                                                     >  1:398480/1‑129 (MQ=255)                        ttACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGc                                                                            >  1:216692/1‑129 (MQ=255)                                                                                      acttacCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCCCTTATAAAATTCa               >  1:148641/1‑128 (MQ=255)                                                                                                  aTTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGAATCCCTTATAAAATTCATATACCtaataaa  >  1:180444/1‑125 (MQ=255)                                                                                                                 |                                                                                                                  TGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTAGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAA  >  NC_000913/2106629‑2106854

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑