Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,188,282	G→A	R978H (CGT→CAT)	rpoC →	RNA polymerase subunit beta'

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,188,282	0	G	A	100.0%	14.0 / NA	9	R978H (CGT→CAT)	rpoC	RNA polymerase subunit beta'
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (2/7);  total (2/7)

CGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGAACAGGTTGCTGGCGGCGAAA  >  NC_000913/4188180‑4188395                                                                                                       |                                                                                                                  cGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCCCAGCAACGTGAAGTCGGTTTTGAACTCCAGCGGTAAACTGGTTTTAACTTCCCATAAACCTAACCTGAAACTGGTcacca                                                                                         >  1:159323/1‑125 (MQ=255)     tgctgAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCTAGCGGTAAACTGGTTATCACTTCCCATAATACTGAACTGAAACTGATCGACGAAtt                                                                                     <  1:175326/129‑1 (MQ=255)      ctcgAAACCCGCATCCAAGGGAAAAACAAAGGTAGAATCAAGCCCAGCAACGGTAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCATAATACTGAACTGAAACTGATCGACGAATTc                                                                                    <  1:167842/126‑1 (MQ=255)                  aTCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCATAATACTGAACTGAAACGGAACGACGAATTCGGTGGTACaaa                                                                         >  1:184720/1‑128 (MQ=255)                        aggAAAAACCAAGGGAGACTCAAGGCCAGGAACGTGAAGTCGGTTGTGAACTCCCGCGGTAAATTGGGTAACACTTCCCATAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGc                                                                  <  1:362317/127‑1 (MQ=255)                                    ggTCGCAACAAGCTCAGAAACGTGAAGTTGGTTGTGAACTCAAGCGGTAAACTGGTTATCACTTCCCATAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCt                                                      <  1:155318/129‑1 (MQ=255)                                         aaaaaaGCCCAGCACCGGGAGGTGGGTTGT‑AACCCCAGAGGTAAACTGGTTATCCCTTCCCATAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGt                                                <  1:76953/125‑1 (MQ=255)                                                                         aCTCCCGGGGGAAACTGGTTAACACCTCCCATAATTATGAACTGAATCCGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGAACAgg                 <  1:183513/129‑1 (MQ=255)                                                                                       ttGGTTATCA‑TTCCCAAAAAACCGAACCTGAACTGGTCGCCAAATTCGGTCGTACGAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGAACAGGTTGCTGGCGGCGaaa  <  1:326468/128‑1 (MQ=255)                                                                                                       |                                                                                                                  CGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAGCGGTAAACTGGTTATCACTTCCCGTAATACTGAACTGAAACTGATCGACGAATTCGGTCGTACTAAAGAAAGCTACAAAGTACCTTACGGTGCGGTACTGGCGAAAGGCGATGGCGAACAGGTTGCTGGCGGCGAAA  >  NC_000913/4188180‑4188395

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑