| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,817,170 | C→A | E112* (GAA→TAA) | yqaB ← | fructose‑1‑phosphatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,817,170 | 0 | C | A | 88.9% | 12.6 / ‑5.2 | 9 | coding (334/567 nt) | yqaB | fructose‑1‑phosphatase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base A (5/3); minor base . (1/0); total (6/3) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTG > NC_000913/2817050‑2817271 | cgcACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCg > 1:274853/1‑129 (MQ=255) cTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATCCCTCAGCGAGGGCGCTTT‑ACATCCCATTCCCTCAGCCACTgggg > 1:471595/1‑127 (MQ=255) gcggggTTTATGGTTTTTGACGGGTTCGGCCGCTACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGcgacg < 1:589179/126‑1 (MQ=255) cgcgGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATCCCTCAGCGGGGGGGCTTAAACCCCCCG‑TCCTAAAGCCATTGGGggaggc > 1:426270/1‑126 (MQ=255) cgGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGcgacga > 1:593613/1‑128 (MQ=255) gtTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCATCAGCGTTGGCGCTTTAACTCCCCGTTCCACAAGCCATTGGGCGCAGACCATG‑CAACttt > 1:461213/1‑128 (MQ=255) cAAAATAATGGCGTAATCCCAGGTTCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTTGGCGACGACCCGGCCAACTTTTCCCCACACAACCAAGAGAAAGGGTTTcac > 1:139152/1‑127 (MQ=255) aaatatGGGGTAAACCCAGGGGCGCCCGCAATGCCCCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGc < 1:312623/125‑1 (MQ=255) cccggaaTGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTAAAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTg < 1:513672/124‑1 (MQ=255) | CGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTG > NC_000913/2817050‑2817271 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |