| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,270,262 | A→C | noncoding (331/377 nt) | rnpB ← | RNase P catalytic RNA component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,270,262 | 0 | A | C | 76.3% | 73.0 / 6.6 | 38 | noncoding (331/377 nt) | rnpB | RNase P catalytic RNA component |
| Reads supporting (aligned to +/- strand): ref base A (4/5); new base C (28/1); total (32/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.35e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATT‑CATCTAGGCCAGC‑AATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACGGGCCGTACCTTATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCC > NC_000913/3270147‑3270369 | gtGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGGCATT‑CCTCTGGgcaagc‑aa > 1:268962/1‑129 (MQ=255) tGCCCCTCCAAAAGCAAAAACCCGCCGAAACGTGTTTTTACGTAAATCCGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATT‑CATCTAGGCCAGC‑AATCGCTCACTGGCTCAagcag < 1:190641/128‑1 (MQ=255) aGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAACCCGGGTTCTGCAGTGGACAGCCATC‑AATCAAGGCACGA‑AACGGTCCACTGGCT‑AAGAAGCCTCACCgggagtc > 1:226814/1‑125 (MQ=9) aaaaaCCCCCCGATGGGGTGTTTTTGGTAAATCTGTTGAATATGGCCCTAAGGCCGGTTTCTGT‑GTGGACAGTCATT‑CATCTAGGCCAGC‑AATTCCTCACCGGCCCAAGCAGCCTACCCGGGTTCAGTa < 1:464525/129‑1 (MQ=255) aaaaaCCCCCCGAAAGGGGTTTTAACGTAAAAC‑GGGGAAACTGGCCCGTAAGCCCGGGTCTGGCGTTGACAGTCATT‑CCTCTAGGCCAGC‑AATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTa < 1:373857/129‑1 (MQ=255) aaaCCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATT‑CATCTAGGCCAGC‑AAGCGCTCACTGGCCCAAGCAACTCACCCGGGTTCAgacg > 1:552458/1‑126 (MQ=37) ttttACGTAAAGCAGGTGAAACCTACCTATAAGCCGGGTTTTGTCGTTGACAGTCATT‑CATCTAGGCCAGC‑AATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACGGGCCGTACCTTATGAAc < 1:588132/129‑1 (MQ=255) tCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATT‑CATCTAGGCCAGC‑AATCGCTCACTGGCTCAAGCAGCTTCCCCGGGTTCAGTCGGGGCCGACCCTTTTAAACCCCAATTGGGc > 1:239859/1‑129 (MQ=255) gccGATAAGCCGGGTTCTGTCGTGGACAGTCATT‑CCTCTAGGCGTTA‑CGTCACCGCAACGCTCAAGCAACCTACCCGGATCCAACGCGGGCCGTGCC‑AAAGGATCCCTATTTGGTCTTGCCCCAGGTgg > 1:306052/2‑129 (MQ=255) gccGATAAGCCGGGTTCTGTCGTGGACAGTCATT‑CCTCTAGGCGTTA‑CGTCACCGCAACGCTCAAGCAACCTACCCGGATCCAACGCGGGCCGCGCCAAAGGATCCCC‑ATTTGGTCTTGCTCCAGGTgg > 1:333879/2‑129 (MQ=255) gccGATAAGCCGGGTTCTGTCGTGGACAGTCATT‑CCTCTAGGCGTTA‑CGTCACCGCAACGCTCAAGCAACCTACCCGGATCCAACGCGGGCCGCGCC‑AAAGGATCCCTATTTGGTCTTGCTCCgggggg > 1:318680/2‑129 (MQ=255) gccGATAAGCCGGGTTCTGTCGTGGACAGTCATT‑CCTCTAGGCGTTA‑CGTCACCGCAACGCTCAAGCAACCTACCCGGATCCAACGCGGGCCGCGCC‑AAAGGATCCCTATTTGGTCTTGCTCCAGGTgg > 1:339503/2‑129 (MQ=255) gccGATAAGCCGGGTTCTGTCGTGGACAGTCATT‑CCTCTAGGCGTTA‑CGTCACCGCAACGCTCAAGCAACCTACCCGGATCCAACGCGGGCCGCGCC‑AAAGGACCCCTATTTGGCTTTGCCCCAGGTgg > 1:290254/2‑129 (MQ=255) ccGATAAGCCGGGTTCTGTCGTGGACAGTCATT‑Cctct > 1:330577/1‑38 (MQ=255) ccGATAAGCCGGGTTCTGTCGTGGACAGTCATT‑CCTCTAGGCGTTA‑CGTCACCGCAACGCTCAAGCAACCTACCCGGATCCAATGCGGGCCGCGCC‑AAAGGATCCCTATTTGGTCTTGCTCCAGGCggg > 1:321679/1‑128 (MQ=255) cGATAAGCCGGGTTCTGTCGTGGACAGTCATT‑CCTCTGGGCGTTA‑CGTCACCGCAACGCTCAAGCAACCTACCCGGATCCAACGCGGGCCGCGCCAAAGGAA‑CCCTATTTGGCCTTGCCCCAGTTgggg > 1:167534/1‑127 (MQ=255) cGATAAGCCGGGTTCTGTCGTGGACAGTCATT‑CCTCTAGGCGTTA‑CGTCACCGCAACGCTCAAGCAACCTACCCGGATCCAACGCGGGCCGGGCCCAAGGAA‑CCCTATTTGGCTTTGCCCCCGGTgggg > 1:214747/1‑127 (MQ=255) cGATAAGCCGGGTTCTGTCGTGGACAGTCATT‑CCTCTAGGCGTTA‑CGTCACCGCAACGCTCAAGCAACCTACCCGGATCCAACGCGGGCCGCGCCAAAGGATCCCC‑ATTTGGTCTTGCTCCAGGTgggg > 1:326517/1‑127 (MQ=255) cGATAAGCCGGGTTCTGTCGTGGACAGTCATT‑CCTCTAGGCGTTA‑CGTCACCGCAACGCTCAAGCAACCTACCCGGATCCAACGCGGGCCGCGCCAAAGGAA‑CCCTATTTGGGCTTGCCCCAGGTgggg > 1:306958/1‑127 (MQ=255) cGATAAGCCGGGTTCTGTCGTGGACAGTCATT‑CCTCTAGGCGTTA‑CGTCACCGCAACGCTCAAGCAACCTACCCGGATCCAACGCGGGCCGCGCC‑CAAGGATCCCTATTTGGTCTTGCTCCAGGTgggg > 1:336663/1‑127 (MQ=255) cGATAAGCCGGGTTCTGTCGTGGACAGTCATT‑CCTCTAGGCGTTA‑CGTCACCGCAACGCTCAAGCAACCTACCCGGATCCAACGCGGGCCGCGCC‑AAAGGATCCCTATTTGGTCTTGCTCCAGGTgggg > 1:318512/1‑127 (MQ=255) cGATAAGCCGGGTTCTGTCGTGGACAGTCATT‑CCTCTAGGCGTTA‑CGTCACCGCAACGCTCAAGCAACCTACCCGGATCCAACGCGGGCCGCGCC‑AAAGGATCCCTATTTGGTCTTGCTCCAGGTgggg > 1:549085/1‑127 (MQ=255) cGATAAGCCGGGTTCTGTCGTGGACAGTCATT‑CCTCTAGGCGTTA‑CGTCACCGCAACGCTCAAGCAACCTACCCGGATCCAACGCGGGCCGCGCC‑AAAGGATCCCTATTTGGTCTTGCTCCAGGTgggg > 1:240318/1‑127 (MQ=255) cGATAAGCCGGGTTCTGTCGTGGACAGTCATT‑CCTCTAGGCGTTA‑CGTCACCGCAACGCTCAAGCAACCTACCCGGATCCAACGCGGGCCGCGCC‑AAAGGATCCCTATTTGGTCTTGCTCCAGGTgggg > 1:275492/1‑127 (MQ=255) cGATAAGCCGGGTTCTGTCGTGGACAGTCATT‑CCTCTAGGCGTTA‑CGTCACCGCAACGCTCAAGCAACCTACCCGGATCCAACGCGGGCCGCGCC‑AAAGGATCCCTATTTGGTCTTGCTCCAGGTgggg > 1:318720/1‑127 (MQ=255) cGATAAGCCGGGTTCTGTCGTGGACAGTCATT‑CCTCTAGGCGTTA‑CGTCACCGCAACGCTCAAGCAACCTACCCGGATCCAACGCGGGCCGCGCC‑AAAGGATCCCTATTTGGTCTTGCTCCAGGTgggg > 1:292450/1‑127 (MQ=255) cGATAAGCCGGGTTCTGTCGTGGACAGTCATT‑CCTCTAGGCGTTA‑CGTCACCGCAACGCTCAAGCAACCTACCCGGATCCAACGCGGGCCGCGCC‑AAAGGATCCCTATTTGGCCTTGCTCCAGGTgggg > 1:335486/1‑127 (MQ=255) cGATAAGCCGGGTTCTGTCGTGGACAGTCATT‑CCTCTAGGCGTTA‑CGTCACCGCAACGCTCAAGCAACCTACCCGGATCCAACGCGGGCCGCGCC‑AAAGGACCCCTATTTGGTCTTGCTCCAGGTgggg > 1:324410/1‑127 (MQ=255) cGATAAGCCGGGTTCTGTCGTGGACAGTCATT‑CCTCTAGGCGTTA‑CGTCACCGCAACGCTCAAGCAACCTACCCGGATCCAACGCGGGCCGCGCC‑AAAGGACCCCTATTTGGCCTTGCCCCAGGTgggg > 1:290601/1‑127 (MQ=255) cGATAAGCCGGGTTCTGTCGTGGACAGTCATT‑CCTCTAGGCGTTA‑CGTCACCGCAACGCTCAAGCAACCTACCCGGATCCAACCCGGGCCGCGCC‑AAAGGATCCCTATTTGGTCTTGCTCCAGGTgggg > 1:310122/1‑127 (MQ=255) cGATAAGCCGGGTTCTGTCGTGGACAGTCATT‑CCTCTAGGCGTTA‑CGTCACCGCAACGCTCAAGCAACCTACCCGGATCCAACCCGGGCCGCGCC‑AAAGGATCCCTATTTGGCCTTGCCCCCGGTgggg > 1:325192/1‑127 (MQ=255) gATAAGCCGGGTTCTGTCGTGGACAGTCATT‑CCTCTAGGCGTTA‑CGTCACCGCCACGCTCAAGCAACCTACCCGGATCCAACGCGGGCCGCGCC‑AAAGGATCCCTATTTGGTCTTGCTCCAGGGGGGGt > 1:293068/1‑129 (MQ=255) gATAAGCCGGGTTCTGTCGTGGACAGTCATT‑CCTCTAGGCGTTA‑CGTCACCGCAACGCTCAAGCAACCTACCCGGATCCAACGCGGGCCGCGCCAAAGGATCCCC‑ATTTGGTCTTGCTCCAGGTGGGGt > 1:332582/1‑129 (MQ=255) gATAAGCCGGGTTCTGTCGTGGACAGTCATT‑CCTCTAGGCGTTA‑CGTCACCGCAACGCTCAAGCAACCTACCCGGATCCAACGCGGGCCGCGCCAAAGGAA‑CCCTATTTGGTCTTGCCCCAGGTGGGGt > 1:302789/1‑129 (MQ=255) gATAAGCCGGGTTCTGTCGTGGACAGTCATT‑CCTCTAGGCGTTA‑CGTCACCGCAACGCTCAAGCAACCTACCCGGATCCAACGCGGGCCGCGCC‑AAAGGATCCCTATTTGGTCTTGCTCCAGGTGGGGt > 1:301497/1‑129 (MQ=255) gATAAGCCGGGTTCTGTCGTGGACAGTCATT‑CCTCTAGGCGTTA‑CGTCACCGCAACGCTCAAGCAACCTACCCGGATCCAACGCGGGCCGCGCC‑AAAGGATCCCTATTTGGTCTTGCTCCAGGTGGGGt > 1:242789/1‑129 (MQ=255) cgCCGGGTGTTGTCTGGGAC‑GTCATT‑TAATTAGGCCAGCAAACCGCTC‑CCGGCTCAAGCCGCCCACCCGGGTTCAGTACGGGCCGTACCTTATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTAc < 1:197392/128‑1 (MQ=255) aGCCGGGTTCTTTGTGGGACAGTCATTCCACTTGGGCAAGA‑AATC‑CTCAACGGGCCAAGCCGCCCACCCGGGTTCAGTACGGGCCGTACCTTATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTa < 1:532898/129‑1 (MQ=255) ggTTCTGTCGTGGACAGTCATT‑CATCTAGGCCAGC‑AATCGCCCACGGGCCCAAACGGCCTCCCCGGTTTCAGTGGGGGCTG‑ACCTTTTGAACCCCCTTTTGGCCTT‑CCCCGGGGGGGGTTTTCCGCGcc > 1:7461/1‑129 (MQ=255) | GTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATT‑CATCTAGGCCAGC‑AATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACGGGCCGTACCTTATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCC > NC_000913/3270147‑3270369 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |